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  1. Link

    ampicillin-elisa-kit-ab285227.pdf

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Microbiology Antimicrobial Antibiotic
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Ampicillin ELISA Kit (ab285227)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example data
  • Example data

Key features and details

  • Sensitivity: 10 ng/ml
  • Range: 5 ng/ml - 625 ng/ml
  • Sample type: Milk, Serum, Tissue, Urine
  • Assay type: Quantitative

Overview

  • Product name

    Ampicillin ELISA Kit
  • Sample type

    Milk, Urine, Serum, Tissue
  • Assay type

    Quantitative
  • Sensitivity

    10 ng/ml
  • Range

    5 ng/ml - 625 ng/ml
  • Assay duration

    Multiple steps standard assay
  • Product overview

    Ampicillin ELISA Kit (ab285227, E4350) is a competitive-based ELISA that can detect ampicillin in milk, urine, tissues and serum. It can detect and quantify broad range of ampicillin (5 – 625 ng/ml) within 90 minutes.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components Identifier 1 x 96 tests
    10X Wash buffer NM 1 x 50ml
    Ampicillin Standard Yellow cap 2 vials
    Antibody NM/Red cap 1 x 7ml
    Conjugate Buffer NM/Green 1 x 7.5ml
    ELISA Microplate 1 unit
    Extraction Solution Brown cap 1 x 2ml
    HRP-Conjugate Stock Blue cap 1 x 25µl
    Plate sealers 4 units
    Sample Diluent NM cap 1 x 20ml
    Standard Buffer WM cap 1 x 40ml
    Stop Solution 1 x 10ml
    TMB Substrate Amber cap 1 x 10ml
  • Research areas

    • Microbiology
    • Antimicrobial
    • Antibiotic
  • Relevance

    Ampicillin is a semisynthetic antibiotic, a member of the penicillin family of antibiotics. To extend the usefulness of the penicillins to the treatment of infections caused by gram negative rods, the broad spectrum penicillins (ampicillin, amoxicillin, carbenicillin, and ticarcillin) were developed. It is created by attaching acid radicals to the central structure of 6 aminopenicillanic acid. Similar in action to penicillin G, the most widely used natural penicillin ampicillin (or alpha aminobenzylpenicillin) is more stable in stomach acids and therefore may be given orally; it is also more active against certain strains of bacteria. It is used regularly to treat common urinary tract infections, some respiratory infections, and bacterial meningitis in children, although many strains of Hemophilus influenzae (the most common cause of bacterial meningitis) are now resistant to ampicillin, and it commonly is used along with other drugs.

Images

  • Example data
    Example data

    Ampicillin standard curve.

    This standard curve is for demonstration only. A standard curve must be run with each assay.

  • Example data
    Example data

    Spike recovery experiment: Milk, human serum and urine samples were assayed with and without spike (20 ng/ml) and showed 80-100% recovery.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab285227? Please let us know so that we can cite the reference in this datasheet.

ab285227 has not yet been referenced specifically in any publications.

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