Western blot abreview for Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Loading amount
40 µg
Specification
heart
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TTBS

Secondary antibody

Dilution
1/1000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa

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Submitted Dec 20 2011

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