Western blot abreview for Anti-AMPK alpha 1 + AMPK alpha 2 [34.2] antibody

Excellent
Abreviews
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (10% gel, Tris-Glycine SDS Running Buffer)
Sample
Mouse Cell lysate - whole cell (3T3-L1 adipocytes)
Specification
3T3-L1 adipocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Blotto Buffer
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
BM Chemiluminescence Western Blotting Su
Bands
Specific: 65 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
3T3-L1 whole cell lysate

Additional data

Additional Notes
Transfer membrane: 0.2 um Nitrocellulose Lane 1: 3T3-L1 whole cell lysate I had similar results with C2C12 myotubes (mouse).

Dr. Roger Mcleod

Verified customer

Submitted Jun 23 2014

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