Western blot abreview for Anti-AMPK alpha 1 + AMPK alpha 2 antibody [34.2]

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Human Cell lysate - whole cell (HCT116 human colon carcinoma cell line)
Specification
HCT116 human colon carcinoma cell line
Treatment
Control (Ctl) and 1 mM Hydroxyurea (HU) for 24 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in PBS-T buffer
Dilution
1/5000

Secondary antibody

Name
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 65 kDa
Exposure
2 minute(s) and 0 second(s)

Additional data

Additional Notes
This antibody gives many unspecific signals if it is used in lower dilutions than 1:5000.

Mr. Christian Marx

Verified customer

Submitted Aug 22 2014

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