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    ampk-alpha-1-antibody-2b7-ab110036.pdf

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Validated using a knockout cell line

Anti-AMPK alpha 1 antibody [2B7] (ab110036)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (12)

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Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

Key features and details

  • Mouse monoclonal [2B7] to AMPK alpha 1
  • Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Primary
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Anti-AMPK alpha 1 + AMPK alpha 2 antibody [EPR19549] (ab207442)

View more associated products

Overview

  • Product name

    Anti-AMPK alpha 1 antibody [2B7]
    See all AMPK alpha 1 primary antibodies
  • Description

    Mouse monoclonal [2B7] to AMPK alpha 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.

  • Positive control

    • Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells WB: RAW 264.7, Mouse Liver, Neuro2A cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    2B7
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • KO cell lines

    • Mouse PRKAA1 knockout RAW 264.7 cell line (ab280055)
  • KO cell lysates

    • Mouse PRKAA1 knockout RAW 264.7 cell lysate (ab280114)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 1 protein (ab202156)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF
1/200 - 1/1000.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF
1/200 - 1/1000.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13131 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5562 Human
    • Entrez Gene: 105787 Mouse
    • Entrez Gene: 65248 Rat
    • Omim: 602739 Human
    • SwissProt: Q13131 Human
    • SwissProt: Q5EG47 Mouse
    • SwissProt: P54645 Rat
    • Unigene: 43322 Human
    • Unigene: 207004 Mouse
    • Unigene: 87789 Rat
    see all
  • Alternative names

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Images

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1 : Wild-type RAW 264.7 cell lysate
    Lane 2 : PRKAA1 knockout RAW 264.7 cell lysate
    Lane 3 : Mouse Liver cell lysate
    Lane 4 : Neuro2A cell lysate

    Performed under reducing conditions.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    False colour image of Western blot: Anti-AMPK alpha 1 antibody [2B7] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110036 was shown to bind specifically to AMPK alpha 1. A band was observed at 64 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in PRKAA1 knockout cell line ab280055 (knockout cell lysate ab280114). To generate this image, wild-type and PRKAA1 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : HeLa cell lysate.
    Lane 3 : HepG2 cell lysate.
    Lane 4 : MCF-7 cell lysate.
    Lane 5 : Cos7 cell lysate.
    Lane 6 : NIH3T3 cell lysate.
    Lane 7 : K562 cell lysate.
    Lane 8 : HEK293 cell lysate.
    Lane 9 : PC-12 cell lysate.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    The proposed dilution of ab110036 is 1/10000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (12)

Publishing research using ab110036? Please let us know so that we can cite the reference in this datasheet.

ab110036 has been referenced in 12 publications.

  • Fan H  et al. Combined intake of blueberry juice and probiotics ameliorate mitochondrial dysfunction by activating SIRT1 in alcoholic fatty liver disease. Nutr Metab (Lond) 18:50 (2021). PubMed: 33971886
  • Li X  et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol 11:73 (2020). PubMed: 32116781
  • Yang Q  et al. Prkaa1 Metabolically Regulates Monocyte/Macrophage Recruitment and Viability in Diet-Induced Murine Metabolic Disorders. Front Cell Dev Biol 8:611354 (2020). PubMed: 33511118
  • Xiong ZS  et al. Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms. Mol Med Rep 20:887-894 (2019). PubMed: 31173255
  • Zhu A  et al. Long non-coding RNA H19 down-regulates miR-181a to facilitate endothelial angiogenic function. Artif Cells Nanomed Biotechnol 47:2698-2705 (2019). PubMed: 31267802
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Question

Inquiry: Dear Abcam, My question is about the specificity of AMPK alpha1 antibody (2B7, catalog no: ab110036). Is there any evidence (ie. Western) whether this antibody does or does not cross-react with AMPK alpha2? Thank you.

Read More

Abcam community

Verified customer

Asked on Apr 24 2012

Answer

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer two other products against AMPK alpha 2 which may satisfy your requirement however. According to the information available to us and on the datasheet, Ab3759 does not react withAMPK alpha 2 (https://www.abcam.com/AMPK-alpha-1-antibody-ab3759.html).

Ab31958 has not been tested for cross reactivity however a BLAST of the immunogen sequence did not reveal appropriate homology with AMPK alpha 1 to suggest cross reactivity (https://www.abcam.com/AMPK-alpha-1-antibody-ab31958.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Abcam Scientific Support

Answered on Apr 24 2012

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