Anti-AMPK alpha 1 antibody [2B7] (ab110036)

Submit a review Q&A (1)References (7)

Overview

  • Product name

    Anti-AMPK alpha 1 antibody [2B7]
    See all AMPK alpha 1 primary antibodies

  • Description

    Mouse monoclonal [2B7] to AMPK alpha 1

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Monkey

  • Immunogen

    Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.

  • Positive control

    • Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells.

Applications

Our Abpromise guarantee covers the use of ab110036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Images

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : HeLa cell lysate.
    Lane 3 : HepG2 cell lysate.
    Lane 4 : MCF-7 cell lysate.
    Lane 5 : Cos7 cell lysate.
    Lane 6 : NIH3T3 cell lysate.
    Lane 7 : K562 cell lysate.
    Lane 8 : HEK293 cell lysate.
    Lane 9 : PC-12 cell lysate.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    The proposed dilution of ab110036 is 1/10000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.

References

This product has been referenced in:

  • Xiong ZS  et al. Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms. Mol Med Rep 20:887-894 (2019). Read more (PubMed: 31173255) »
  • Zhao J  et al. Inhibition of CCL19 benefits non-alcoholic fatty liver disease by inhibiting TLR4/NF-?B-p65 signaling. Mol Med Rep 18:4635-4642 (2018). Read more (PubMed: 30221732) »
See all 7 Publications for this product

Publishing research using ab110036? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Inquiry: Dear Abcam, My question is about the specificity of AMPK alpha1 antibody (2B7, catalog no: ab110036). Is there any evidence (ie. Western) whether this antibody does or does not cross-react with AMPK alpha2? Thank you.

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Answer

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer two other products against AMPK alpha 2 which may satisfy your requirement however. According to the information available to us and on the datasheet, Ab3759 does not react withAMPK alpha 2 (https://www.abcam.com/AMPK-alpha-1-antibody-ab3759.html).

Ab31958 has not been tested for cross reactivity however a BLAST of the immunogen sequence did not reveal appropriate homology with AMPK alpha 1 to suggest cross reactivity (https://www.abcam.com/AMPK-alpha-1-antibody-ab31958.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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