Anti-AMPK alpha 1 antibody [2B7] (ab110036)

Submit a review Q&A (1)References (10)
Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)

Key features and details

  • Mouse monoclonal [2B7] to AMPK alpha 1
  • Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

Overview

  • Product name

    Anti-AMPK alpha 1 antibody [2B7]
    See all AMPK alpha 1 primary antibodies

  • Description

    Mouse monoclonal [2B7] to AMPK alpha 1

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.

  • Positive control

    • Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Rat
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF
1/200 - 1/1000.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF
1/200 - 1/1000.
ELISA
1/10000.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Images

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : HeLa cell lysate.
    Lane 3 : HepG2 cell lysate.
    Lane 4 : MCF-7 cell lysate.
    Lane 5 : Cos7 cell lysate.
    Lane 6 : NIH3T3 cell lysate.
    Lane 7 : K562 cell lysate.
    Lane 8 : HEK293 cell lysate.
    Lane 9 : PC-12 cell lysate.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    The proposed dilution of ab110036 is 1/10000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.

References (10)

Publishing research using ab110036? Please let us know so that we can cite the reference in this datasheet.

ab110036 has been referenced in 10 publications.

  • Li X  et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol 11:73 (2020). PubMed: 32116781
  • Xiong ZS  et al. Effect of metformin on cell proliferation, apoptosis, migration and invasion in A172 glioma cells and its mechanisms. Mol Med Rep 20:887-894 (2019). PubMed: 31173255
  • Zhu A  et al. Long non-coding RNA H19 down-regulates miR-181a to facilitate endothelial angiogenic function. Artif Cells Nanomed Biotechnol 47:2698-2705 (2019). PubMed: 31267802
  • Zhao J  et al. Inhibition of CCL19 benefits non-alcoholic fatty liver disease by inhibiting TLR4/NF-?B-p65 signaling. Mol Med Rep 18:4635-4642 (2018). PubMed: 30221732
  • Yang Q  et al. PRKAA1/AMPKa1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nat Commun 9:4667 (2018). PubMed: 30405100
  • Chowdhary S  et al. Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo. Sci Rep 7:5502 (2017). WB, IHC-P ; Drosophila melanogaster . PubMed: 28710464
  • Almabrouk TAM  et al. Deletion of AMPKa1 attenuates the anticontractile effect of perivascular adipose tissue (PVAT) and reduces adiponectin release. Br J Pharmacol 174:3398-3410 (2017). PubMed: 27668984
  • Shin HJ  et al. AMPK-SKP2-CARM1 signalling cascade in transcriptional regulation of autophagy. Nature 534:553-7 (2016). PubMed: 27309807
  • Garvey SM  et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiol Rep 3:N/A (2015). WB ; Rat . PubMed: 25716928
  • Marin TL  et al. Identification of AMP-activated protein kinase targets by a consensus sequence search of the proteome. BMC Syst Biol 9:13 (2015). WB . PubMed: 25890336

Customer reviews and Q&As

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Question

Inquiry: Dear Abcam, My question is about the specificity of AMPK alpha1 antibody (2B7, catalog no: ab110036). Is there any evidence (ie. Western) whether this antibody does or does not cross-react with AMPK alpha2? Thank you.

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Answer

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer two other products against AMPK alpha 2 which may satisfy your requirement however. According to the information available to us and on the datasheet, Ab3759 does not react withAMPK alpha 2 (https://www.abcam.com/AMPK-alpha-1-antibody-ab3759.html).

Ab31958 has not been tested for cross reactivity however a BLAST of the immunogen sequence did not reveal appropriate homology with AMPK alpha 1 to suggest cross reactivity (https://www.abcam.com/AMPK-alpha-1-antibody-ab31958.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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