Overview

  • Product name
    Anti-AMPK alpha 1 antibody [2B7]
    See all AMPK alpha 1 primary antibodies
  • Description
    Mouse monoclonal [2B7] to AMPK alpha 1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IF, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Monkey
  • Immunogen

    Purified recombinant fragment of Human AMPK alpha 1 expressed in E. Coli.

  • Positive control
    • Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH3T3, K562, HEK293 and PC-12 cell lysates. Ovarian cancer and brain tissues. NTERA-2 and PC-2 cells.

Applications

Our Abpromise guarantee covers the use of ab110036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 64 kDa.
ICC/IF 1/200 - 1/1000.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/1000.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Images

  • Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Western blot - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    All lanes : Anti-AMPK alpha 1 antibody [2B7] (ab110036) at 1/500 dilution

    Lane 1 : Jurkat cell lysate.
    Lane 2 : HeLa cell lysate.
    Lane 3 : HepG2 cell lysate.
    Lane 4 : MCF-7 cell lysate.
    Lane 5 : Cos7 cell lysate.
    Lane 6 : NIH3T3 cell lysate.
    Lane 7 : K562 cell lysate.
    Lane 8 : HEK293 cell lysate.
    Lane 9 : PC-12 cell lysate.

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ELISA - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    The proposed dilution of ab110036 is 1/10000.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded ovarian cancer by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in NTERA-2 cells (green) by Immunofluorescence. The nuclei are stained with DRAQ5 fluorescent DNA dye (blue) and the Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
  • Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Flow Cytometry - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 used in Flow cytometric analysis of PC-2 cells (green). The negative control is indicated in purple.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [2B7] (ab110036)
    ab110036 at 1/200 dilution staining AMPK alpha 1 in paraffin-embedded brain tissues by Immunohistochemistry.

References

This product has been referenced in:
  • Chowdhary S  et al. Analysis of mitochondrial organization and function in the Drosophila blastoderm embryo. Sci Rep 7:5502 (2017). WB, IHC-P ; Drosophila melanogaster . Read more (PubMed: 28710464) »
  • Garvey SM  et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats. Physiol Rep 3:N/A (2015). WB ; Rat . Read more (PubMed: 25716928) »

See all 3 Publications for this product

Publishing research using ab110036? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1 Review or Q&A

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Votes: Highest First

Inquiry: Dear Abcam, My question is about the specificity of AMPK alpha1 antibody (2B7, catalog no: ab110036). Is there any evidence (ie. Western) whether this antibody does or does not cross-react with AMPK alpha2? Thank you.

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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