• Product name

    Anti-AMPK alpha 1 antibody
    See all AMPK alpha 1 primary antibodies
  • Description

    Rabbit polyclonal to AMPK alpha 1
  • Host species

  • Tested applications

    Suitable for: IHC-P, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus
  • Immunogen

    Synthetic peptide (Human) - which represented a portion of AMP activated protein Kinase (AMPK), alpha 1 catalytic subunit encoded within exon 8.

  • Positive control

    • HepG2 whole cell lysate (ab7900) can be used as a positive control in WB. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal pancreas It also gave a positive result in PANC1 cell line in IF/ICC


Our Abpromise guarantee covers the use of ab3759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 2-8 µg/mg of lysate.
WB 1/500 - 1/5000. Predicted molecular weight: 63 kDa.


  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all


  • Samples: Extracts from

    1. Bovine aortic endothelial cells

    2. Rat aortic smooth muscle cells

    3. HepG2 cells

    4. Human aortic endothelial cells

    Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).


    Samples: Extracts from

    1. Bovine aortic endothelial cells

    2. Rat aortic smooth muscle cells

    3. HepG2 cells

    4. Human aortic endothelial cells

    Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB.
  • IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab3759 stained PANC1 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3759, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Huang Q  et al. Incremental effect of liraglutide on traditional insulin injections in rats with type 2 diabetes mellitus by maintaining glycolipid metabolism and cardiovascular function. Exp Ther Med 17:1863-1869 (2019). Read more (PubMed: 30783461) »
  • Lee MS  et al. Loss of the E3 ubiquitin ligase MKRN1 represses diet-induced metabolic syndrome through AMPK activation. Nat Commun 9:3404 (2018). Read more (PubMed: 30143610) »
See all 16 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer two other products against AMPK alpha 2 which may satisfy your requirement however. According to the information available to us and on the datasheet, Ab3759 does not react withAMPK alpha 2 (https://www.abcam.com/AMPK-alpha-1-antibody-ab3759.html).

Ab31958 has not been tested for cross reactivity however a BLAST of the immunogen sequence did not reveal appropriate homology with AMPK alpha 1 to suggest cross reactivity (https://www.abcam.com/AMPK-alpha-1-antibody-ab31958.html).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. Our source for ab3759 and ab3760 has told me that the antibodies do not cross-react with the homologs. If you have any additional questions, please contact us again.

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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