Overview

  • Product name

    Anti-AMPK alpha 1 (phospho S487) antibody
    See all AMPK alpha 1 primary antibodies
  • Description

    Rabbit polyclonal to AMPK alpha 1 (phospho S487)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, surrounding phosphorylation site of serine 487 (

    S-G-S(p)-V-S

    ) derived from Human AMPK alpha 1 (NP_006242.5).

  • Positive control

    • HUVEC cell extract treated with UV, HeLa cells, Human colon carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab131357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 64 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

Images

  • All lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution

    Lane 1 : HUVEC cell extract untreated
    Lane 2 : HUVEC cell extract treated with UV

    Predicted band size: 64 kDa

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labelling AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide.
  • Immunofluorescence of methanol-fixed HeLa cells staining AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/100.

References

This product has been referenced in:

  • Xie M  et al. Trimetazidine combined with exercise improves exercise capacity and anti-fatal stress ability through enhancing mitochondrial quality control. Life Sci 224:157-168 (2019). Read more (PubMed: 30872179) »
  • Yang Y  et al. Mechanical stress protects against osteoarthritis via regulation of the AMPK/NF-?B signaling pathway. J Cell Physiol 234:9156-9167 (2019). Read more (PubMed: 30311192) »
See all 10 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Monkey Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 19 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 19 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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