Anti-AMPK alpha 1 (phospho S487) antibody (ab131357)
Key features and details
- Rabbit polyclonal to AMPK alpha 1 (phospho S487)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 1 (phospho S487) antibody
See all AMPK alpha 1 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 1 (phospho S487) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide conjugated to KLH, surrounding phosphorylation site of serine 487 (
S-G-S(p)-V-S
) derived from Human AMPK alpha 1 (NP_006242.5). -
Positive control
- HUVEC cell extract treated with UV, HeLa cells, Human colon carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab131357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 64 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 65248 Rat
- Omim: 602739 Human
- SwissProt: Q13131 Human
- SwissProt: Q5EG47 Mouse
- SwissProt: P54645 Rat
- Unigene: 43322 Human
see all -
Alternative names
- 5 AMP activated protein kinase alpha 1catalytic subunit antibody
- 5 AMP activated protein kinase catalytic alpha 1 chain antibody
- 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
see all
Images
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Western blot - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357)All lanes : Anti-AMPK alpha 1 (phospho S487) antibody (ab131357) at 1/500 dilution
Lane 1 : HUVEC cell extract untreated
Lane 2 : HUVEC cell extract treated with UV
Predicted band size: 64 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labelling AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/50. Picture on the right was preincubated with blocking peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 (phospho S487) antibody (ab131357)Immunofluorescence of methanol-fixed HeLa cells staining AMPK alpha 1 (phospho S487) with ab131357 antibody at a dilution of 1/100.
Protocols
Datasheets and documents
References (13)
ab131357 has been referenced in 13 publications.
- Du S et al. Long non-coding RNA ANRIL alleviates H2O2-induced injury by up-regulating microRNA-21 in human lens epithelial cells. Aging (Albany NY) 12:6543-6557 (2020). PubMed: 32310822
- Xie M et al. Trimetazidine combined with exercise improves exercise capacity and anti-fatal stress ability through enhancing mitochondrial quality control. Life Sci 224:157-168 (2019). PubMed: 30872179
- Yang Y et al. Mechanical stress protects against osteoarthritis via regulation of the AMPK/NF-?B signaling pathway. J Cell Physiol 234:9156-9167 (2019). PubMed: 30311192
- Gao S et al. Protective effects of salvianolic acid B against hydrogen peroxide-induced apoptosis of human umbilical vein endothelial cells and underlying mechanisms. Int J Mol Med 44:457-468 (2019). PubMed: 31173197
- Zhang L et al. Isatin inhibits the invasion of human neuroblastoma SH-SY5Y cells, based on microarray analysis. Mol Med Rep 20:1700-1706 (2019). PubMed: 31257543
