Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920)
Key features and details
- Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody -
Description
Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. This phospho site has 100% homology to AMPK alpha 2 (phospho T172)
Database link: Q13131 -
Positive control
- WB: C2C12 cells treated by Oligomycin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Notes |
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WB
1/500 - 1/2000. |
Target
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
Database links
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- SwissProt: Q5EG47 Mouse
- SwissProt: Q8BRK8 Mouse
- Unigene: 207004 Mouse
- Unigene: 48638 Mouse
Images
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All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920) at 1/2000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : Treated C2C12 cell lysate, cells were treated by Oligomycin (0.5uM) for 30 minutes.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Exposure time: 1 secondBlocking buffer: 3% BSA.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab194920 has been referenced in 6 publications.
- Huang CC et al. Metformin: a novel promising option for fertility preservation during cyclophosphamide-based chemotherapy. Mol Hum Reprod 27:N/A (2021). PubMed: 33543290
- Fan H et al. Combined intake of blueberry juice and probiotics ameliorate mitochondrial dysfunction by activating SIRT1 in alcoholic fatty liver disease. Nutr Metab (Lond) 18:50 (2021). PubMed: 33971886
- An Y et al. SIRT1 inhibits chemoresistance and cancer stemness of gastric cancer by initiating an AMPK/FOXO3 positive feedback loop. Cell Death Dis 11:115 (2020). PubMed: 32051395
- Li X et al. Puerarin Attenuates Diabetic Nephropathy by Promoting Autophagy in Podocytes. Front Physiol 11:73 (2020). PubMed: 32116781
- Kan J et al. S100A16, a novel lipogenesis promoting factor in livers of mice and hepatocytes in vitro. J Cell Physiol 234:21395-21406 (2019). PubMed: 31069793
- Li Y et al. AMP-activated protein kinase stimulates osteoblast differentiation and mineralization through autophagy induction. Int J Mol Med 41:2535-2544 (2018). PubMed: 29484369