Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody -
Description
Rabbit polyclonal to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. This phospho site has 100% homology to AMPK alpha 2 (phospho T172)
Database link: Q13131 -
Positive control
- WB: C2C12 cells treated by Oligomycin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab194920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. |
Target
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Cellular localization
AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters. -
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Omim: 600497 Human
- Omim: 602739 Human
- SwissProt: P54646 Human
- SwissProt: Q13131 Human
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Images
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Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920)All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody (ab194920) at 1/2000 dilution
Lane 1 : C2C12 cell lysate
Lane 2 : Treated C2C12 cell lysate, cells were treated by Oligomycin (0.5uM) for 30 minutes.
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Exposure time: 1 secondBlocking buffer: 3% BSA.
Protocols
References
This product has been referenced in:
- Li Y et al. AMP-activated protein kinase stimulates osteoblast differentiation and mineralization through autophagy induction. Int J Mol Med 41:2535-2544 (2018). Read more (PubMed: 29484369) »
