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RecombinantRabMAb

Recombinant Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Reviews (4) Submit a question References (79)
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
  • Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Drosophila melanogaster

Conjugates logo Related conjugates and formulations

Carrier Free Carrier Free

Overview

  • Product name

    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
    See all AMPK alpha 1+AMPK alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Drosophila melanogaster

  • Immunogen

    Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. Also within AMPK alpha 2 (phospho T172).

  • Positive control

    • WB: HEK-293 and MOLT-4 whole cell lysates; Mouse heart lysate; Rat brain lysate;

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).
Application notes
Is unsuitable for Flow Cyt.

Target

Images

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution

    Lane 1 : HEK293 (human embryonic kidney) whole cell lysates
    Lane 2 : HEK293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
    Lane 3 : HEK293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa


    Exposure time: 15 seconds


    Blocking and dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 64 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 64 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 64 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

    Lane 1 : Untreated HEK293 cell lysate
    Lane 2 : HEK293 cell lysate treated with Lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 64 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : Lysate of 293 cells treated with lambda phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 64 kDa

  • OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)
    Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

References (79)

Publishing research using ab133448? Please let us know so that we can cite the reference in this datasheet.

ab133448 has been referenced in 79 publications.

  • Hao J  et al. Discovery of natural adenosine monophosphate-activated protein kinase activators through virtual screening and activity verification studies. Mol Med Rep 23:N/A (2021). PubMed: 33495803
  • Zhang C  et al. Isoalantolactone inhibits pancreatic cancer proliferation by regulation of PI3K and Wnt signal pathway. PLoS One 16:e0247752 (2021). PubMed: 33661942
  • Hu Y & Ma X Icariin Treatment Protects Against Gentamicin-Induced Ototoxicity via Activation of the AMPK-SIRT3 Pathway. Front Pharmacol 12:620741 (2021). PubMed: 33679402
  • Yang B  et al. MOTS-c interacts synergistically with exercise intervention to regulate PGC-1a expression, attenuate insulin resistance and enhance glucose metabolism in mice via AMPK signaling pathway. Biochim Biophys Acta Mol Basis Dis 1867:166126 (2021). PubMed: 33722744
  • Vidimce J  et al. Mitochondrial Function, Fatty Acid Metabolism, and Body Composition in the Hyperbilirubinemic Gunn Rat. Front Pharmacol 12:586715 (2021). PubMed: 33762933
View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

 Poor
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (tadpole limb)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
15 µg
Specification
tadpole limb
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

DR. Gufa Lin

Verified customer

Submitted Aug 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer 1x
Permeabilization
No
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

DR. Liad Hinden

Verified customer

Submitted Sep 20 2016

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Horse Cell lysate - whole cell (jejunum epithelium)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
jejunum epithelium
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Jul 18 2016

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)
Specification
0-4hr embryos
Treatment
control shRNA and SNF1A shRNA embryos
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

DR. Richelle Sopko

Verified customer

Submitted Dec 16 2013

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