Name: Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
Brand: RabMAb
SKU: ab133448
Rating: 4 (4 reviews)

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Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
Suitable for: WB
Reacts with: Mouse, Rat, Human, Drosophila melanogaster

Product name

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]
See all AMPK alpha 1+AMPK alpha 2 primary antibodies
Description

Rabbit monoclonal [EPR5683] to AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)
Host species

Rabbit
Specificity

This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.
Tested applications

Suitable for: WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human, Drosophila melanogaster
Immunogen

Synthetic peptide within Human AMPK alpha 1 (phospho T183). The exact sequence is proprietary. Also within AMPK alpha 2 (phospho T172).
Positive control
- WB: HEK-293 and MOLT-4 whole cell lysates; Mouse heart lysate; Rat brain lysate;
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Dissociation constant (K_D)

K_D = 1.22 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5683
Isotype

IgG
Research areas

Alternative Versions
- Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] - Azide free (ab240058)
- Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 antibody [EPR5683] - BSA and Azide free (ab271892)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HEK-293 whole cell lysate (ab7902)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(3)	1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 62,63 kDa (predicted molecular weight: 64 kDa).

Application notes

Is unsuitable for Flow Cyt.

Cellular localization

AMPK alpha 2: Cytoplasm. Nucleus. In response to stress, recruited by p53/TP53 to specific promoters.
Database links
- Entrez Gene: 5562 Human
- Entrez Gene: 5563 Human
- Entrez Gene: 105787 Mouse
- Entrez Gene: 108079 Mouse
- Entrez Gene: 65248 Rat
- Entrez Gene: 78975 Rat
- Omim: 600497 Human
- Omim: 602739 Human
- SwissProt: P54646 Human
- SwissProt: Q13131 Human
- SwissProt: Q5EG47 Mouse
- SwissProt: Q8BRK8 Mouse
- SwissProt: P54645 Rat
- SwissProt: Q09137 Rat
- Unigene: 43322 Human
- Unigene: 437039 Human
- Unigene: 207004 Mouse
- Unigene: 48638 Mouse
- Unigene: 64583 Rat
- Unigene: 87789 Rat
see all

Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/2000 dilution

Lane 1 : HEK293 (human embryonic kidney) whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 phospho pT183 and AMPK alpha 2 phospho pT172 peptides
Lane 3 : HEK293 (human embryonic kidney) whole cell lysates plus AMPK alpha 1 and AMPK alpha 2 non-phospho peptides

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Fruit fly lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 64 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

Lane 1 : Mouse heart lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 64 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified) + Molt-4 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 64 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (purified)

Lane 1 : Untreated HEK293 cell lysate
Lane 2 : HEK293 cell lysate treated with Lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 64 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

All lanes : Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448) at 1/1000 dilution (unpurified)

Lane 1 : 293 cell lysate
Lane 2 : Lysate of 293 cells treated with lambda phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 64 kDa
OI-RD Scanning - Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] (ab133448)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab133448? Please let us know so that we can cite the reference in this datasheet.

ab133448 has been referenced in 56 publications.

Khorraminejad-Shirazi M et al. AICAR and nicotinamide treatment synergistically augment the proliferation and attenuate senescence-associated changes in mesenchymal stromal cells. Stem Cell Res Ther 11:45 (2020). PubMed: 32014016
Gong H et al. Nrf2-SHP Cascade-Mediated STAT3 Inactivation Contributes to AMPK-Driven Protection Against Endotoxic Inflammation. Front Immunol 11:414 (2020). PubMed: 32210977
Luan M et al. TIPRL, a Novel Tumor Suppressor, Suppresses Cell Migration, and Invasion Through Regulating AMPK/mTOR Signaling Pathway in Gastric Cancer. Front Oncol 10:1062 (2020). PubMed: 32719745
Xia T et al. ColXV Aggravates Adipocyte Apoptosis by Facilitating Abnormal Extracellular Matrix Remodeling in Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32024006
Gao Z et al. The Protective Effects of Imperatorin on Acetaminophen Overdose-Induced Acute Liver Injury. Oxid Med Cell Longev 2020:8026838 (2020). PubMed: 32454943

View all Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Xenopus laevis Cell lysate - whole cell (tadpole limb)

Gel Running Conditions

Non-reduced Denaturing (10%)

Loading amount

15 µg

Specification

tadpole limb

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gufa Lin

Verified customer

Submitted Aug 28 2017

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer 1x

Permeabilization

Specification

kidney

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

Dr. Liad Hinden

Verified customer

Submitted Sep 20 2016

Application

Western blot

Sample

Horse Cell lysate - whole cell (jejunum epithelium)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Specification

jejunum epithelium

Blocking step

Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 18 2016

Application

Western blot

Loading amount

30 µg

Gel Running Conditions

Reduced Denaturing (4-12%)

Sample

Fruit fly (Drosophila melanogaster) Tissue lysate - whole (0-4hr embryos)

Specification

0-4hr embryos

Treatment

control shRNA and SNF1A shRNA embryos

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Richelle Sopko

Verified customer

Submitted Dec 16 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Cellular localization

Database links

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (56)

Customer reviews and Q&As

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) [EPR5683] antibody

Dissociation constant (K_D)