Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Excellent
Abreviews
Application
Western blot
Sample
Horse Cell lysate - whole cell (jejunum epithelium)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
jejunum epithelium
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/7500
Name
Non-Abcam antibody was used: donkey-anti-rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: ~ 62 kDa

Additional data

Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback We are pleased that ab133448 is working well for you in horse, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in horse which will help us assess how well the antibody works in this species.

Abcam user community

Verified customer

Submitted Jul 18 2016

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