Western blot abreview for Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683]

Poor
Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (tadpole limb)
Gel Running Conditions
Non-reduced Denaturing (10%)
Loading amount
15 µg
Specification
tadpole limb
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: HRP goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)

Additional data

Abcam response
Thank you for having tested ab133448 in an untested species (xenopus) and your participation to our AbTrial program. We welcome any further feedbacks in Xenopus using AbTrial: www.abcam.com/abtrial

Gufa Lin

Verified customer

Submitted Aug 28 2017

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