Western blot abreview for Anti-AMPK alpha 2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse skeletal muscle cell)
Loading amount
25 µg
Specification
mouse skeletal muscle cell
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% skim milk+ 0.05% TTBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 63 kDa Non-specific: 70 kDa

Abcam user community

Verified customer

Submitted Nov 24 2009

Sign up