Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AMPK alpha 2 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Thymus)
Specification
Thymus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50

Additional data

Additional Notes
ab3760 presents in both the nuclear and the cytoplasm in mouse thymus and skin.

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Submitted Mar 25 2010

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