Western blot abreview for Anti-AMPK alpha 2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat heart cell (cardiac myocyte) lysates prepared)
Loading amount
20 µg
Specification
Rat heart cell (cardiac myocyte) lysates prepared
Treatment
I/R plus treatment of urocortin (Ucn1) or I/R plus treatment with Ucn2
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in tbs tween

Secondary antibody

Name
Non-Abcam antibody was used: secondary anti-rabbit HRP 1:300
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
Pierce west pico supersignal
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 58 kDa

Prof. Paul Townsend

Verified customer

Submitted Mar 24 2010

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