Western blot abreview for Anti-AMPK alpha 2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary hepatocytes (liver))
Loading amount
24 µg
Specification
Primary hepatocytes (liver)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule) Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 63 kDa Non-specific: 48 kDa

Additional data

Additional Notes
Lane 1: Hepatocytes cultured in Williams Medium E for 9 hours;
Lane 2: Hepatocytes cultured in Williams Medium E for 57 hours;
Lane 3: Hepatocytes cultured in Williams Medium E for 57 hours with 0.1% DMSO.

*Hepatocytes were lysed using np-40 buffer, cell lysate was loaded 1:1 with 2X Tris-Gly SDS reducing sample buffer, 24 ug sample added to each well.

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Submitted Apr 02 2012

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