Western blot abreview for Anti-AMPK alpha 2 antibody

Excellent
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Schwann cells)
Loading amount
20 µg
Specification
Schwann cells
Gel Running Conditions
Reduced Denaturing (10% SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 63 kDa

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Submitted Jun 26 2012

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