Western blot abreview for Anti-AMPK alpha 2 antibody

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (squeletal muscle, adipose tissus)
Gel Running Conditions
Reduced Denaturing (gradient gel 4-15%)
Loading amount
25 µg
Specification
squeletal muscle, adipose tissus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-tween 0.05 %
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Amersham ECL Rabbit IgG, HRP-linked whole Ab (from
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa

Additional data

Additional Notes
Pretty Good antibody. it worked very well in mouse squeletal muscles (gastrocnemius and soleus). We observed some extra bands in mouse adipose tissus. it Worked also in human hepg2 cells line. Lane 1 : Mouse 1 squeletal muscle Lane 2 : Mouse 2 squeletal muscle Lane 3 : Mouse 3 squeletal muscle

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Submitted Feb 22 2017

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