Overview

  • Product name

    Anti-AMPK gamma 1 antibody
    See all AMPK gamma 1 primary antibodies
  • Description

    Rabbit polyclonal to AMPK gamma 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Cow, Pig
  • Immunogen

    Recombinant full length protein corresponding to Human AMPK gamma 1 aa 1-331.
    Database link: P54619

  • Positive control

    • WB: Rat liver tissue. IHC-P: Human testis tissue. ICC/IF: PC-3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
  • Sequence similarities

    Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
    Contains 4 CBS domains.
  • Domain

    The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
    The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
  • Post-translational
    modifications

    Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5' AMP activated protein kinase gamma 1 subunit antibody
    • 5' AMP activated protein kinase subunit gamma 1 antibody
    • 5''-AMP-activated protein kinase subunit gamma-1 antibody
    • AAKG1_HUMAN antibody
    • AMP activated protein kinase noncatalytic gamma 1 subunit antibody
    • AMPK gamma 1 chain antibody
    • AMPK gamma1 antibody
    • AMPK subunit gamma-1 antibody
    • AMPKg antibody
    • MGC8666 antibody
    • PRKAG 1 antibody
    • PRKAG1 antibody
    • Protein kinase AMP activated gamma 1 non catalytic subunit antibody
    • protein kinase, AMP-activated, noncatalytic gamma-1 antibody
    see all

Images

  • Anti-AMPK gamma 1 antibody (ab223116) at 1/500 dilution + Rat liver lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa

  • PC-3 (human prostate adenocarcinoma cell line) cells stained for AMPK gamma 1 (green) using ab223116 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Paraffin-embedded human testis tissue stained for AMPK gamma 1 using ab223116 at 1/100 dilution in immunohistochemical analysis.

References

ab223116 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223116.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up