Western blot abreview for Anti-AMPK gamma 1 antibody [Y307]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
100000 cells
Specification
HeLa
Treatment
AMPK gamma1 siRNA for 36hrs
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% skim milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: Ant iRabbit IgG HRP linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 38 kDa

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Submitted Feb 11 2010

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