Recombinant
RabMAb

Recombinant Anti-AMPK gamma 1 antibody [Y308] - BSA and Azide free (ab239818)

Overview

  • Product name

    Anti-AMPK gamma 1 antibody [Y308] - BSA and Azide free
    See all AMPK gamma 1 primary antibodies
  • Description

    Rabbit monoclonal [Y308] to AMPK gamma 1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
    Unsuitable for: ICC or IHC
  • Species reactivity

    Reacts with: Mouse, Rat, Human, African green monkey
  • Immunogen

    Synthetic peptide within Human AMPK gamma 1 aa 300-400 (C terminal). The exact sequence is proprietary.

  • General notes

    Ab239818 is the carrier-free version of ab32508. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239818 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab239818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent concentration. PubMed: 23612997
  • Application notes
    Is unsuitable for ICC or IHC.
  • Target

    • Function

      AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
    • Sequence similarities

      Belongs to the 5'-AMP-activated protein kinase gamma subunit family.
      Contains 4 CBS domains.
    • Domain

      The AMPK pseudosubstrate motif resembles the sequence around sites phosphorylated on target proteins of AMPK, except the presence of a non-phosphorylatable residue in place of Ser. In the absence of AMP this pseudosubstrate sequence may bind to the active site groove on the alpha subunit (PRKAA1 or PRKAA2), preventing phosphorylation by the upstream activating kinase STK11/LKB1.
      The CBS domains mediate binding to AMP, ADP and ATP. 2 sites bind either AMP or ATP, whereas a third site contains a tightly bound AMP that does not exchange. Under physiological conditions AMPK mainly exists in its inactive form in complex with ATP, which is much more abundant than AMP.
    • Post-translational
      modifications

      Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5' AMP activated protein kinase gamma 1 subunit antibody
      • 5' AMP activated protein kinase subunit gamma 1 antibody
      • 5''-AMP-activated protein kinase subunit gamma-1 antibody
      • AAKG1_HUMAN antibody
      • AMP activated protein kinase noncatalytic gamma 1 subunit antibody
      • AMPK gamma 1 chain antibody
      • AMPK gamma1 antibody
      • AMPK subunit gamma-1 antibody
      • AMPKg antibody
      • MGC8666 antibody
      • PRKAG 1 antibody
      • PRKAG1 antibody
      • Protein kinase AMP activated gamma 1 non catalytic subunit antibody
      • protein kinase, AMP-activated, noncatalytic gamma-1 antibody
      see all

    Images

    • AMPK gamma 1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10ug of Rabbit monoclonal [Y308] to AMPK gamma 1and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Jurkat whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32508. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Bands: 37kDa: AMPK gamma 1.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32508).

    References

    ab239818 has not yet been referenced specifically in any publications.

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