Recombinant Anti-AMPS antibody [EPR10746(B)] (ab154872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10746(B)] to AMPS
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-AMPS antibody [EPR10746(B)]
See all AMPS primary antibodies -
Description
Rabbit monoclonal [EPR10746(B)] to AMPS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human AMPS.
Database link: P30566 -
Positive control
- WB: C6, Raw 264.7, NIH/3T3, HeLa, HepG2 and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. IP: NIH/3T3
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10746(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
|
|
IP |
1/40.
|
|
ICC/IF |
1/250 - 1/500.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
IP
1/40. |
ICC/IF
1/250 - 1/500. |
Target
-
Tissue specificity
Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1. -
Pathway
Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2. -
Involvement in disease
Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency) [MIM:103050]. ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado). Most children display marked psychomotor delay, often accompanied by epilepsy or autistic features, or both, although some patients may be less profoundly retarded. Occasionally, growth retardation and muscular wasting are also present. -
Sequence similarities
Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily. - Information by UniProt
-
Database links
- Entrez Gene: 158 Human
- Entrez Gene: 11564 Mouse
- Entrez Gene: 315150 Rat
- Omim: 608222 Human
- SwissProt: P30566 Human
- SwissProt: P54822 Mouse
- Unigene: 75527 Human
- Unigene: 38151 Mouse
-
Alternative names
- Adenylosuccinase antibody
- Adenylosuccinate lyase antibody
- ADSL antibody
see all
Images
-
All lanes : Anti-AMPS antibody [EPR10746(B)] (ab154872) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lane 6 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 55 kDa -
Immunofluorescence analysis of HeLa cells labeling AMPS with ab154872 at 1/250 dilution.
-
AMPS was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Lane 1: NIH/3T3 whole cell extract, 10 µg (Input).
Lane 2: ab154872 IP in NIH/3T3 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 1 seconds
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154872 has not yet been referenced specifically in any publications.