Overview

  • Product name

    Anti-AMPS antibody [EPR10746(B)]
    See all AMPS primary antibodies
  • Description

    Rabbit monoclonal [EPR10746(B)] to AMPS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human AMPS.
    Database link: P30566

  • Positive control

    • WB: C6, Raw 264.7, NIH/3T3, HeLa, HepG2 and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. IP: NIH/3T3
  • General notes

     

     

    Previously labelled as Adenylosuccinate Lyase. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP 1/40.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Tissue specificity

      Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1.
    • Pathway

      Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.
    • Involvement in disease

      Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency) [MIM:103050]. ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado). Most children display marked psychomotor delay, often accompanied by epilepsy or autistic features, or both, although some patients may be less profoundly retarded. Occasionally, growth retardation and muscular wasting are also present.
    • Sequence similarities

      Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.
    • Information by UniProt
    • Database links

    • Alternative names

      • Adenylosuccinase antibody
      • Adenylosuccinate lyase antibody
      • ADSL antibody
      • AMPS antibody
      • ASase antibody
      • ASL antibody
      • OTTHUMP00000199172 antibody
      • OTTHUMP00000199173 antibody
      • PUR8_HUMAN antibody
      see all

    Images

    • All lanes : Anti-AMPS antibody [EPR10746(B)] (ab154872) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : Raw 264.7 cell lysate
      Lane 3 : NIH/3T3 cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 55 kDa

    • Immunofluorescence analysis of HeLa cells labeling AMPS with ab154872 at 1/250 dilution.

    • AMPS was immunoprecipitated from 1mg of NIH/3T3 (Mouse embyro fibroblast cells) whole cell extract with ab154872 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab154872 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

      Lane 1: NIH/3T3 whole cell extract, 10 µg (Input).

      Lane 2: ab154872 IP in NIH/3T3 whole cell extract.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154872 in NIH/3T3 whole cell extract.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 seconds

       

    References

    ab154872 has not yet been referenced specifically in any publications.

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