Recombinant
RabMAb

Recombinant Anti-AMPS antibody [EPR10747(B)] (ab154182)

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Overview

  • Product name

    Anti-AMPS antibody [EPR10747(B)]
    See all AMPS primary antibodies

  • Description

    Rabbit monoclonal [EPR10747(B)] to AMPS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human AMPS. The exact sequence is proprietary. The antibody does not recognize human ADSL isoform 2.

  • Positive control

    • HeLa, Jurkat, HepG2 and HCT-116 cell lysates, Human thyroid gland carcinoma tissue, permeabilized HeLa cells

  • General notes

    Previously labelled as Adenylosuccinate Lyase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab154182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Tissue specificity

      Ubiquitously expressed. Both isoforms are produced by all tissues. Isoform 2 is 10-fold less abundant than isoform 1.

    • Pathway

      Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.
      Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.

    • Involvement in disease

      Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency) [MIM:103050]. ADSL deficiency is an autosomal recessive disorder characterized by the accumulation in the body fluids of succinylaminoimidazole-carboxamide riboside (SAICA-riboside) and succinyladenosine (S-Ado). Most children display marked psychomotor delay, often accompanied by epilepsy or autistic features, or both, although some patients may be less profoundly retarded. Occasionally, growth retardation and muscular wasting are also present.

    • Sequence similarities

      Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.
    • Information by UniProt

    • Database links

      • Alternative names

        • Adenylosuccinase antibody
        • Adenylosuccinate lyase antibody
        • ADSL antibody
        • AMPS antibody
        • ASase antibody
        • ASL antibody
        • OTTHUMP00000199172 antibody
        • OTTHUMP00000199173 antibody
        • PUR8_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue labeling AMPS with ab154182 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154182 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Flow Cytometry - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Flow cytometric analysis of permeabilized HeLa cells with ab154182 at 1/100 dilution(red) or a rabbit IgG (negative) (green).
      • Western blot - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Western blot - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        All lanes : Anti-AMPS antibody [EPR10747(B)] (ab154182) at 1/1000 dilution

        Lane 1 : HeLa cell lysates
        Lane 2 : Jurkat cell lysates
        Lane 3 : HepG2 cell lysates
        Lane 4 : HCT-116 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 55 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154182 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154182 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154182 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPS antibody [EPR10747(B)] (ab154182)

        Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154182 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

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