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Cell Biology Cell Cycle Cell Division Cytokinesis
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-AMSH antibody [EPR4361] (ab108301)

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Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
  • Anti-AMSH antibody [EPR4361] (ab108301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4361] to AMSH
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human AMSH protein (ab139803)
Knockout
Product image
Human STAMBP (AMSH) knockout HeLa cell lysate (ab258213)

View more associated products

Overview

  • Product name

    Anti-AMSH antibody [EPR4361]
    See all AMSH primary antibodies
  • Description

    Rabbit monoclonal [EPR4361] to AMSH
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat cell lysate, HeLa cell lysate, HAP1 cell lysate, Raji cell lysate, SW480 cell lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4361
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Alternative Versions

    • Anti-AMSH antibody [EPR4361] - BSA and Azide free (ab247622)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human STAMBP (AMSH) knockout HeLa cell line (ab265249)
  • KO cell lysates

    • Human STAMBP (AMSH) knockout HeLa cell lysate (ab258213)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • SW480 whole cell lysate (ab3957)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human AMSH protein (ab139803)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.

Target

  • Function

    Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The JAMM motif is essential for the protease activity.
  • Post-translational
    modifications

    Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • Cellular localization

    Nucleus. Membrane. Cytoplasm. Early endosome.
  • Target information above from: UniProt accession O95630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10617 Human
    • Entrez Gene: 70527 Mouse
    • Entrez Gene: 171565 Rat
    • Omim: 606247 Human
    • SwissProt: O95630 Human
    • SwissProt: Q9CQ26 Mouse
    • SwissProt: Q8R424 Rat
    • Unigene: 469018 Human
    • Unigene: 657598 Human
    • Unigene: 32801 Mouse
    • Unigene: 14662 Rat
    see all
  • Alternative names

    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Images

  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : STAM Binding Protein knockout HeLa lysate
    Lane 3 : HAP1 lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab108301 Anti-STAMBP antibody was shown to specifically react with STAM Binding Protein in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265249 (knockout cell lysate ab258213) was used. Wild-type and STAM Binding Protein knockout samples were subjected to SDS-PAGE. ab108301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : AMSH knockout HAP1 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 48 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE.  Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa

  • Anti-AMSH antibody [EPR4361] (ab108301)
    Anti-AMSH antibody [EPR4361] (ab108301)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab108301? Please let us know so that we can cite the reference in this datasheet.

ab108301 has not yet been referenced specifically in any publications.

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