Product nameAnti-AMSH antibody [EPR4361]
See all AMSH primary antibodies
DescriptionRabbit monoclonal [EPR4361] to AMSH
Tested applicationsSuitable for: WB, ICCmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human AMSH aa 150-250. The exact sequence is proprietary.
- Jurkat cell lysate, HeLa cell lysate, Raji cell lysate, SW480 cell lysate
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 48 kDa.|
|ICC||1/100 - 1/250.|
FunctionZinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the peptidase M67C family.
Contains 1 MPN (JAB/Mov34) domain.
DomainThe JAMM motif is essential for the protease activity.
modificationsPhosphorylated after BMP type I receptor activation.
Ubiquitinated by SMURF2 in the presence of RNF11.
Cellular localizationNucleus. Membrane. Cytoplasm. Early endosome.
- Information by UniProt
- AMSH antibody
- Associated molecule with the SH3 domain of STAM antibody
- Endosome associated ubiquitin isopeptidase antibody
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: AMSH knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE. Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
ab108301 has not yet been referenced specifically in any publications.