Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-AMSH antibody [EPR4361] (ab108301)

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Overview

  • Product name

    Anti-AMSH antibody [EPR4361]
    See all AMSH primary antibodies

  • Description

    Rabbit monoclonal [EPR4361] to AMSH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICCmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human AMSH aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Jurkat cell lysate, HeLa cell lysate, Raji cell lysate, SW480 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
ICC 1/100 - 1/250.

Target

  • Function

    Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.

  • Domain

    The JAMM motif is essential for the protease activity.

  • Post-translational
    modifications

    Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.

  • Cellular localization

    Nucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Images

  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    Western blot - Anti-AMSH antibody [EPR4361] (ab108301)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: AMSH knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: Jurkat whole cell lysate (20 µg)
    Lane 4: HeLa whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE.  Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    Western blot - Anti-AMSH antibody [EPR4361] (ab108301)
    All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa

References

ab108301 has not yet been referenced specifically in any publications.

Publishing research using ab108301? Please let us know so that we can cite the reference in this datasheet.

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