Recombinant Anti-AMSH antibody [EPR4361] (ab108301)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-AMSH antibody [EPR4361]
See all AMSH primary antibodies -
Description
Rabbit monoclonal [EPR4361] to AMSH -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human AMSH aa 150-250. The exact sequence is proprietary.
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Positive control
- Jurkat cell lysate, HeLa cell lysate, Raji cell lysate, SW480 cell lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4361 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| ICC | 1/100 - 1/250. |
Target
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Function
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the peptidase M67C family.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The JAMM motif is essential for the protease activity. -
Post-translational
modificationsPhosphorylated after BMP type I receptor activation.
Ubiquitinated by SMURF2 in the presence of RNF11. -
Cellular localization
Nucleus. Membrane. Cytoplasm. Early endosome. - Information by UniProt
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Database links
- Entrez Gene: 10617 Human
- Entrez Gene: 70527 Mouse
- Entrez Gene: 171565 Rat
- Omim: 606247 Human
- SwissProt: O95630 Human
- SwissProt: Q9CQ26 Mouse
- SwissProt: Q8R424 Rat
- Unigene: 469018 Human
see all -
Alternative names
- AMSH antibody
- Associated molecule with the SH3 domain of STAM antibody
- Endosome associated ubiquitin isopeptidase antibody
see all
Images
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-281802-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: AMSH knockout HAP1 whole cell lysate (20 µg)
Lane 3: Jurkat whole cell lysate (20 µg)
Lane 4: HeLa whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE. Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-120817-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa
Datasheets and documents
References
ab108301 has not yet been referenced specifically in any publications.
