Recombinant Anti-AMSH antibody [EPR4361] (ab108301)
![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-387259-anti-stambp-antibody-western-blot-hela-hap1-jurkat-lysates.jpg "Western blot - Anti-AMSH antibody [EPR4361] (ab108301)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4361] to AMSH
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-AMSH antibody [EPR4361]
See all AMSH primary antibodies -
Description
Rabbit monoclonal [EPR4361] to AMSH -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat cell lysate, HeLa cell lysate, HAP1 cell lysate, Raji cell lysate, SW480 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4361 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
Target
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Function
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the peptidase M67C family.
Contains 1 MPN (JAB/Mov34) domain. -
Domain
The JAMM motif is essential for the protease activity. -
Post-translational
modificationsPhosphorylated after BMP type I receptor activation.
Ubiquitinated by SMURF2 in the presence of RNF11. -
Cellular localization
Nucleus. Membrane. Cytoplasm. Early endosome. - Information by UniProt
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Database links
- Entrez Gene: 10617 Human
- Entrez Gene: 70527 Mouse
- Entrez Gene: 171565 Rat
- Omim: 606247 Human
- SwissProt: O95630 Human
- SwissProt: Q9CQ26 Mouse
- SwissProt: Q8R424 Rat
- Unigene: 469018 Human
see all -
Alternative names
- AMSH antibody
- Associated molecule with the SH3 domain of STAM antibody
- Endosome associated ubiquitin isopeptidase antibody
see all
Images
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Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : STAM Binding Protein knockout HeLa lysate
Lane 3 : HAP1 lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDaLanes 1-4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.
ab108301 Anti-STAMBP antibody was shown to specifically react with STAM Binding Protein in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265249 (knockout cell lysate ab258213) was used. Wild-type and STAM Binding Protein knockout samples were subjected to SDS-PAGE. ab108301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-281802-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AMSH knockout HAP1 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 48 kDaLanes 1 - 4: Merged signal (red and green). Green - ab108301 observed at 48 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108301 was shown to specifically react with AMSH in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when AMSH knockout samples were examined. Wild-type and AMSH knockout samples were subjected to SDS-PAGE. Ab108301 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-120817-anti-amsh-antibody-epr4361-western-blot.jpg)
Western blot - Anti-AMSH antibody [EPR4361] (ab108301)All lanes : Anti-AMSH antibody [EPR4361] (ab108301) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 48 kDa -
![Anti-AMSH antibody [EPR4361] (ab108301)](https://www.abcam.com/ps/products/108/ab108301/Images/ab108301-4-benefits-of-recombinant-antibodies.png)
Anti-AMSH antibody [EPR4361] (ab108301)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab108301 has not yet been referenced specifically in any publications.