Overview

  • Product name

    Anti-AMSH antibody - N-terminal
    See all AMSH primary antibodies
  • Description

    Rabbit polyclonal to AMSH - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to N terminal amino acids 1-302 of the Human AMSH protein (O95630).

  • Positive control

    • WB: PC-3, U87-MG, NIH-3T3, JC, and BCL-1 cell lysates. IHC-P: Breast ca.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab151475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains (By similarity). Functions at the endosome and is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF. Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The JAMM motif is essential for the protease activity.
  • Post-translational
    modifications

    Phosphorylated after BMP type I receptor activation.
    Ubiquitinated by SMURF2 in the presence of RNF11.
  • Cellular localization

    Nucleus. Membrane. Cytoplasm. Early endosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • AMSH antibody
    • Associated molecule with the SH3 domain of STAM antibody
    • Endosome associated ubiquitin isopeptidase antibody
    • Endosome-associated ubiquitin isopeptidase antibody
    • MGC126516 antibody
    • MGC126518 antibody
    • MICCAP antibody
    • STABP_HUMAN antibody
    • STAM binding protein antibody
    • STAM-binding protein antibody
    • Stambp antibody
    see all

Images

  • Immunohistochemistry analysis of paraffin-embedded Human breast ca tissue labeling AMSH with ab151475 at 1/500.
  • All lanes : Anti-AMSH antibody - N-terminal (ab151475) at 1/1000 dilution

    Lane 1 : PC-3 Whole cell lysate
    Lane 2 : U87-MG Whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa



    10% SDS PAGE
  • All lanes : Anti-AMSH antibody - N-terminal (ab151475) at 1/1000 dilution

    Lane 1 : NIH-3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 48 kDa



    10% SDS PAGE

References

ab151475 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151475.
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