Key features and details
- Rabbit polyclonal to AMSH-LP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-AMSH-LP antibody
See all AMSH-LP primary antibodies
DescriptionRabbit polyclonal to AMSH-LP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Rhesus monkey
Recombinant fragment within Human AMSH-LP (internal sequence). The exact sequence is proprietary.
Database link: Q96FJ0
- WB: IMR32 whole cell lysate. IHC-P: CL1-5 xenograft tissue. ICC/IF: HeLa cells.
Previously labelled as STAMBPL1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 50 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionZinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the peptidase M67C family.
Contains 1 MPN (JAB/Mov34) domain.
DomainThe JAMM motif is essential for the protease activity.
- Information by UniProt
- ALMalpha antibody
- AMSH FP antibody
- AMSH LP antibody
Anti-AMSH-LP antibody (ab229144) at 1/2000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS-PAGE gel.
Paraffin-embedded CL1-5 xenograft tissue stained for AMSH-LP using ab229144 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AMSH-LP (green) using ab229144 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229144 has been referenced in 1 publication.
- Yu DJ et al. STAMBPL1 knockdown has antitumour effects on gastric cancer biological activities. Oncol Lett 18:4421-4428 (2019). PubMed: 31611951