Overview

  • Product name

  • Description

    Rabbit polyclonal to AMSH-LP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human AMSH-LP (internal sequence). The exact sequence is proprietary.
    Database link: Q96FJ0

  • Positive control

    • WB: IMR32 whole cell lysate. IHC-P: CL1-5 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Previously labelled as STAMBPL1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab229144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the peptidase M67C family.
    Contains 1 MPN (JAB/Mov34) domain.
  • Domain

    The JAMM motif is essential for the protease activity.
  • Information by UniProt
  • Database links

  • Alternative names

    • ALMalpha antibody
    • AMSH FP antibody
    • AMSH LP antibody
    • AMSH-like protease antibody
    • AMSH-LP antibody
    • AMSHFP antibody
    • AMSHLP antibody
    • Associated molecule with the SH3 domain of STAM (AMSH) like protein antibody
    • FLJ31524 antibody
    • OTTHUMP00000020055 antibody
    • STALP_HUMAN antibody
    • STAM binding protein like 1 antibody
    • STAM-binding protein-like 1 antibody
    • STAMBPL1 antibody
    see all

Images

  • Anti-AMSH-LP antibody (ab229144) at 1/2000 dilution + IMR32 (human neuroblast cell line) whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded CL1-5 xenograft tissue stained for AMSH-LP using ab229144 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AMSH-LP (green) using ab229144 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229144.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up