Overview

  • Product name
    Anti-AMT antibody - N-terminal
    See all AMT primary antibodies
  • Description
    Rabbit polyclonal to AMT - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human AMT aa 25-54 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P48728

  • Positive control
    • WB: HepG2 cell lysate. IHC-P: Human hepatocarcinoma tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 44 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Anti-AMT antibody - N-terminal (ab175545) at 1/1000 dilution + HepG2 cell lysate at 35 µg

    Predicted band size: 44 kDa

  • Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma labeling AMT at 1/50 dilution with ab175545, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

  • Flow cytometry analysis of HepG2 cells (bottom histogram) labeling AMT at 1/10 dilution compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175545 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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