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Synthetic peptide within Human AMT aa 25-54 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P48728
Our Abpromise guarantee covers the use of ab175545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 44 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Immunohistochemistry analysis of formalin-fixed and paraffin-embedded Human hepatocarcinoma labeling AMT at 1/50 dilution with ab175545, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of HepG2 cells (bottom histogram) labeling AMT at 1/10 dilution compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175545 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"