• Product name

    Amylase Assay Kit (Colorimetric)
  • Detection method

  • Sample type

    Cell culture supernatant, Urine, Serum, Tissue Extracts, Cell culture media
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    > 0.2 mU/well
  • Assay time

    0h 40m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Amylase Assay Kit (Colorimetric) ab102523 detects activity of α-amylase through a two-step reaction.

    In the amylase assay protocol, alpha-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by alpha-glucosidase. This causes the release of a chromophore that can then be measured at OD = 405 nm.

    The assay can detect α-amylase content as low as 0.2 mU.

    Amylase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader every 2-3 min for 30-60 min


  • Notes

    Amylases are enzymes that break starch down to sugar molecules. α-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). α-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. 

  • Platform

    Microplate reader



  • Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline or one of three drugs. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

    There were no statistically significant changes in plasma amylase activity in mice that were administered with two of the drugs vs mice administered saline. However, administration of the third to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.

  • Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.

    Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.

  • Sample Test



This product has been referenced in:

  • Murthy P  et al. Enhanced Neutrophil Extracellular Trap Formation in Acute Pancreatitis Contributes to Disease Severity and Is Reduced by Chloroquine. Front Immunol 10:28 (2019). Read more (PubMed: 30733719) »
  • D'Onofrio N  et al. Antioxidant and Anti-Inflammatory Activities of Buffalo Milk d-Valerobetaine. J Agric Food Chem 67:1702-1710 (2019). Read more (PubMed: 30661355) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Amylase activity kits with chicken intestinal samples

Excellent Excellent 5/5 (Ease of Use)
The Amylase Assay Kit (ab102523) used to measure amylase activity in chicken intestinal samples worked well.
However, due to multiple attempts in trying to figure out the right dilution (between 1:50 and 1:400) of fresh samples and due to high individual variation, we managed to test the kits on a very small number of birds (9) since we used all the amylase substrate bottle provided (5ml) and the nitrophenol standard when preparing standard curves. Better to use three dilutions first and test it on a small number of samples, then continue the analysis with the final chosen dilution. It is advisable to have 2 kits as the nitrophenol standard is not enough to prepare at least 2 standard curve. One standard curve consumes 90 microliter of the solution, and the amount provided is 150 microl- It would have been better if it was at least 180 or 200 microliter.

Abcam user community

Verified customer

Submitted Oct 30 2017


I confirm this kit is suitable for measuring mouse serum amylase.

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Thank you for contacting us and for your interest in our products.

I am pleased to confirm that the Amylase Assay Kit (ab102523) is suitable for the use with chicken plasma samples. Please do not hesitate to contact us in case you are experiencing any problems using chicken plasma with this kit.

I hope this is of help. Please do not hesitate to contact us again if you require further information.

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These 2 kit buffers have different components and pH. They cannot be used interchangeably and it is not recommended to perform the extraction with PBS.

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We are not aware of any lipase interference in this assay.
The reaction principle is mentioned on our datasheet for this product. The complete organic reaction is proprietary information. The same goes for the positive control identity and activity as well. Sorry!

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Thank you for contacting us.

The protocol can be found by clicking the following link;


We do not have received any feedback from other researchers so I am sorry we will not be able to share any publication.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry regarding ab102523.

I can confirm that this kit can be used for detecting Amylase in different sample types such as Cell culture supernatant, Urine, Serum, Plasma, Tissue Extracts, Cell culture media - as well as Other biological fluids. This means that it should work in saliva.

If you need any further assistance in the future, please do not hesitate to contact me.

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Thank you for your telephone enquiry this morning.

I can confirm that this kit has not been specifically tested on rat samples, and so regrettably there is no data to provide. However, theoretically it should work in rat and so we can guarantee it for use in this species.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Thank you for contacting us.

Unfortunately, at this time our testing discount program only applies to primary antibodies, proteins, and ELISA kits. I am sorry I could not be more helpful, please let me know if you need any further assistance.

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1-10 of 15 Abreviews or Q&A

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