Overview

  • Product name
    Amylase Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media
  • Assay type
    Quantitative
  • Sensitivity
    > 0.2 mU/well
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Mammals
  • Product overview

    Amylase Assay kit (Colorimetric) (ab102523) detects activity of a-amylase through a two-step reaction. a-Amylase will cleave the substrate ethylidene-pNP-G7 to produce smaller fragments that are eventually modified by a-glucosidase, causing the release of a chromophore that can then be measured at OD = 405 nm. The assay can detect a-amylase content as low as 0.2 mU.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Amylases are enzymes that break starch down to sugar molecules. a-amylase is the major form of amylase found in humans and other mammals as well as an enzyme present in seeds, or in fungi (baker's yeast for instance). a-amylase is a calcium metalloenzyme, completely unable to function in the absence of calcium. In human physiology, both the salivary and pancreatic amylases are major digestive enzymes. Increased enzyme levels in humans are associated with salivary trauma; mumps due to inflammation of the salivary glands, pancreatitis and renal failure. A simple, direct and automation-ready procedure for measuring a-amylase activity is, therefore, very desirable.

  • Platform
    Microplate

Properties

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Amylase activity measured in biological fluids showing activity (mU) per mL of tested sample. Samples were diluted 2 fold.

  • Amylase activity measured in tissue lysates showing activity (mU) per mg of extracted protein.

    Protein concentration for samples varied from 16 mg/mL to 50 mg/mL. Samples were diluted 2 fold.

  • Sample Test
  • Plasma amylase levels were measured (using ab102523) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.

    There were no statistically significant changes in plasma amylase activity in mice that were administered liraglutide or exendin-4 vs mice administered saline. However, administration of sitagliptin to animals on normal diet led to a 1.4-fold increase in amylase activity (p≤0.01, n = 3 per group) and a 1.3- fold increase in mice on a high fat diet (p≤0.01, n = 4 per group).

Protocols

References

This product has been referenced in:
  • Merle NS  et al. Characterization of Renal Injury and Inflammation in an Experimental Model of Intravascular Hemolysis. Front Immunol 9:179 (2018). Read more (PubMed: 29545789) »
  • O'Harte FP  et al. Dogfish glucagon analogues counter hyperglycaemia and enhance both insulin secretion and action in diet-induced obese diabetic mice. Diabetes Obes Metab 18:1013-24 (2016). Read more (PubMed: 27357054) »

See all 4 Publications for this product

Customer reviews and Q&As

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Amylase activity kits with chicken intestinal samples

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The Amylase Assay Kit (ab102523) used to measure amylase activity in chicken intestinal samples worked well.
However, due to multiple attempts in trying to figure out the right dilution (between 1:50 and 1:400) of fresh samples and due to high individual variation, we managed to test the kits on a very small number of birds (9) since we used all the amylase substrate bottle provided (5ml) and the nitrophenol standard when preparing standard curves. Better to use three dilutions first and test it on a small number of samples, then continue the analysis with the final chosen dilution. It is advisable to have 2 kits as the nitrophenol standard is not enough to prepare at least 2 standard curve. One standard curve consumes 90 microliter of the solution, and the amount provided is 150 microl- It would have been better if it was at least 180 or 200 microliter.
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Submitted Oct 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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