Overview

  • Product name
  • Description
    Rabbit polyclonal to Amylin
  • Host species
    Rabbit
  • Specificity
    ab55411 detects endogenous levels of total Amylin protein.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from human Amylin.

  • Positive control
    • Human brain tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: U87-MG

Properties

Applications

Our Abpromise guarantee covers the use of ab55411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

Target

  • Function
    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • Sequence similarities
    Belongs to the calcitonin family.
  • Domain
    The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
  • Post-translational
    modifications
    Amyloid fibrils are degraded by IDE.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amylin antibody
    • DAP antibody
    • Diabetes associated peptide antibody
    • Diabetes-associated peptide antibody
    • IAP antibody
    • IAPP antibody
    • IAPP_HUMAN antibody
    • Insulinoma amyloid peptide antibody
    • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
    • Islet amyloid polypeptide antibody
    see all

Images

  • ICC/IF image of ab55411 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab55411 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab55411 (1/50 dilution), in the presence or absence of the immunizing peptide.

References

ab55411 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.
Concerning ab18018 - there is significant sequence homology between human amylin and that of mouse and rat, but this antibody has only been tested with human samples.
Unfortunately, we do not have any experimental data regarding the cross-reactivity of ab15125 and ab15128 and ab55411 with mouse or rat samples too.
I compared the immunogen for ab15125 with that of the mouse and rat Amylin proteins and the percentage homology for both is 100%. Based on that information ab15125 is most likely not suitable for your application.
Please do not hesitate to contact me with additional questions or concerns.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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