Overview

  • Product name

  • Description

    Rabbit polyclonal to Amylin/DAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Amylin/DAP aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
    Database link: P10997

  • Positive control

    • Pancreas.
  • General notes

     This product was previously labelled as Amylin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab15125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/200 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min before commencing with IHC staining protocol.

Target

  • Function

    Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.
  • Sequence similarities

    Belongs to the calcitonin family.
  • Domain

    The mature protein is largely unstructured in the absence of a cognate ligand, and has a strong tendency to form fibrillar aggregates. Homodimerization may be the first step of amyloid formation.
  • Post-translational
    modifications

    Amyloid fibrils are degraded by IDE.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amylin antibody
    • DAP antibody
    • Diabetes associated peptide antibody
    • Diabetes-associated peptide antibody
    • IAP antibody
    • IAPP antibody
    • IAPP_HUMAN antibody
    • Insulinoma amyloid peptide antibody
    • Islet amyloid polypeptide (diabetes associated peptide; amylin) antibody
    • Islet amyloid polypeptide antibody
    see all

Images

  • ab15125 staining Amylin/DAP in human pancreas by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

References

This product has been referenced in:

  • Martinez-Valbuena I  et al. Interaction of amyloidogenic proteins in pancreatic ß cells from subjects with synucleinopathies. Acta Neuropathol N/A:N/A (2018). IHC-P ; Human . Read more (PubMed: 29536165) »
  • Ramzy A  et al. Insulin-Deficient Mouse ß-Cells Do Not Fully Maturebut Can Be Remedied Through Insulin Replacementby Islet Transplantation. Endocrinology 159:83-102 (2018). Read more (PubMed: 29029025) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.
Concerning ab18018 - there is significant sequence homology between human amylin and that of mouse and rat, but this antibody has only been tested with human samples.
Unfortunately, we do not have any experimental data regarding the cross-reactivity of ab15125 and ab15128 and ab55411 with mouse or rat samples too.
I compared the immunogen for ab15125 with that of the mouse and rat Amylin proteins and the percentage homology for both is 100%. Based on that information ab15125 is most likely not suitable for your application.
Please do not hesitate to contact me with additional questions or concerns.

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