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    amyloid-fibril-antibody-moc78-conformation-specific-ab205341.pdf

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RecombinantRabMAb

Recombinant Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
  • Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [mOC78] to Amyloid Fibril - Conformation-Specific
  • Suitable for: Dot blot, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific
    See all Amyloid Fibril primary antibodies
  • Description

    Rabbit monoclonal [mOC78] to Amyloid Fibril - Conformation-Specific
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Dot Blot: beta Amyloid (Aß) 1-40; beta Amyloid (Aß) 1-42. IHC-P: FFPE Hu Brain Alzheimer.
  • General notes

    This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.

    ab205341  (mOC78) recognizes a conformation-dependent and aggregation-specific discontinuous epitope of beta Amyloid that maps to segments 8-11 (SGYE), 18-24  (VFFAEDV) and 26-32 (SNKGAII) (Hatami et al 2014). It  also recognizes a-synuclein and islet amyloid polypeptide (IAPP) fibrils but not monomers on dot blots. It recognizes a broad range of bands from monomer to high molecular weight with samples of Aß40 and Aß42 on western blots (Hatami et al 2014).  Immunoreactivity on western blots is not changed by boiling the membrane. mOC78 stains plaques and a unique population of intraneuronal and intranuclear amyloid and also stains the center of nascent neuritic plaques where it colocalizes with neuronal chromatin (Pensalfani et al 2014). 

    For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    mOC78
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-Amyloid Fibril antibody [mOC78] - BSA and Azide free (ab251428)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab205341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/6000.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Dot blot
1/6000.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Cellular localization

    Membrane.
  • Database links

    • Entrez Gene: 351 Human
    • GenBank: 9606 Human
    • Omim: 104760 Human
    • SwissProt: P05067 Human
    • Alternative names

      • AAA antibody
      • ABETA antibody
      • ABPP antibody
      • AD1 antibody
      • Alzheimer disease amyloid protein antibody
      • Amyloid beta precursor protein antibody
      • APP antibody
      • APPI antibody
      • Beta amyloid peptide antibody
      • Cerebral vascular amyloid peptide antibody
      • CTFgamma antibody
      • CVAP antibody
      • Peptidase nexin II antibody
      • PN II antibody
      • PN2 antibody
      • PreA4 antibody
      • Protease nexin II antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

      IHC image of Amyloid Fibrillin staining in Human Brain Alzheimer formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab205341, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
      Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

      Dot blot analysis of beta Amyloid labeled with ab205341 at 1/6000 dilution.

      Lane 1: beta Amyloid (Aβ) 1-40;

      Lane 2: beta Amyloid (Aβ) 1-42.

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/30000 was used as secondary antibody.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      Note: Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab205341:

      Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.

      Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.

      Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.

    • Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
      Dot Blot - Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

      Negative control (secondary ab only) Dot blot analysis of beta Amyloid.

      Lane 1: beta Amyloid (Aβ) 1-40;

      Lane 2: beta Amyloid (Aβ) 1-42.

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/30000 was used as secondary antibody.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)
      Anti-Amyloid Fibril antibody [mOC78] - Conformation-Specific (ab205341)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab205341? Please let us know so that we can cite the reference in this datasheet.

    ab205341 has been referenced in 4 publications.

    • Paulus A  et al. Amyloid Structural Changes Studied by Infrared Microspectroscopy in Bigenic Cellular Models of Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33810433
    • Hatami A  et al. Familial Alzheimer's Disease Mutations within the Amyloid Precursor Protein Alter the Aggregation and Conformation of the Amyloid-ß Peptide. J Biol Chem 292:3172-3185 (2017). PubMed: 28049728
    • Hatami A  et al. Monoclonal antibodies against Aß42 fibrils distinguish multiple aggregation state polymorphisms in vitro and in Alzheimer disease brain. J Biol Chem 289:32131-43 (2014). PubMed: 25281743
    • Pensalfini A  et al. Intracellular amyloid and the neuronal origin of Alzheimer neuritic plaques. Neurobiol Dis 71:53-61 (2014). PubMed: 25092575

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