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    amyloid-fibril-antibody-moc87-conformation-specific-ab201062.pdf

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RecombinantRabMAb

Recombinant Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (4)

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Immunohistochemistry - Free Floating - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
  • Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
  • Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [mOC87] to Amyloid Fibril - Conformation-Specific
  • Suitable for: Dot blot, IHC-P, IHC-FrFl
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific
    See all Amyloid Fibril primary antibodies
  • Description

    Rabbit monoclonal [mOC87] to Amyloid Fibril - Conformation-Specific
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-P, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Amyloid Fibril. Amyloid beta 1-42 fibrils were used as the immunogen.
    Database link: P05067

  • Positive control

    • beta Amyloid (Aß) 1-40; beta Amyloid (Aß) 1-42. IHC-P: FFPE human Alzheimer hippocampus tissue sections.
  • General notes

    This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.

    ab201062 recognizes a generic epitope of amyloid fibrils and oligomers that is independent of linear sequence (Hatami et al. 2014). Its reactivity with Aß monomer and oligomers is decreased or eliminated upon thermal denaturation at 100°C of Aß in SDS sample buffer on western blots (Hatami et al. 2014).

    For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    mOC87
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Diseases

Associated products

  • Alternative Versions

    • Anti-Amyloid Fibril antibody [mOC87] - BSA and Azide free (ab251335)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab201062 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
1/8000.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FrFl
Use at an assay dependent concentration.
Notes
Dot blot
1/8000.
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FrFl
Use at an assay dependent concentration.

Target

  • Cellular localization

    Membrane.
  • Database links

    • Entrez Gene: 351 Human
    • Entrez Gene: 11820 Mouse
    • GenBank: 9606 Human
    • Omim: 104760 Human
    • SwissProt: P05067 Human
    • SwissProt: P12023 Mouse
    • Alternative names

      • AAA antibody
      • ABETA antibody
      • ABPP antibody
      • AD1 antibody
      • Alzheimer disease amyloid protein antibody
      • Amyloid beta precursor protein antibody
      • APP antibody
      • APPI antibody
      • Beta amyloid peptide antibody
      • Cerebral vascular amyloid peptide antibody
      • CTFgamma antibody
      • CVAP antibody
      • Peptidase nexin II antibody
      • PN II antibody
      • PN2 antibody
      • PreA4 antibody
      • Protease nexin II antibody
      see all

    Images

    • Immunohistochemistry - Free Floating - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
      Immunohistochemistry - Free Floating - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)Image courtesy of Professor Charles Glabe, UC Irvine

      Immunohistochemical staining of human brain tissue from a patient with a diagnosis of Alzheimers disease, male, 81 years, 5 hour post mortem index, tangle stage 5, plaque stage B, mini mental status exam score 12. Sections were cut using a vibratome. No antigen retrieval was performed. Free floating sections were stained using ab201062 at a dilution of 50 ng/mL. The secondary antibody used was a biotinylated goat anti-rabbit at a dilution of 1/225, which was blocked with normal goat serum. The sample was visualized using ABC solution (1 hour incubation) followed by 1-4 minutes of DAB. The sample was mounted and allowed to dry overnight, followed by dehydration in increasingly concentrated ethanol solutions.

    • Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
      Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

      Dot blot analysis of beta Amyloid 1-42 labeled with ab201062 at 1/8000 dilution.
      Lane 1: beta Amyloid (Aβ) 1-40.
      Lane 2: beta Amyloid (Aβ) 1-42.
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
      Blocking and diluting buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

      Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. Beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab201062:
      Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.
      Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.
      Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

      IHC image of beta Amyloid staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201062, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
      Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

      Negative control (secondary ab only):

      Lane 1: beta Amyloid (Aβ) 1-40.
      Lane 2: beta Amyloid (Aβ) 1-42.
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
      Blocking and diluting buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
      Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab201062? Please let us know so that we can cite the reference in this datasheet.

    ab201062 has been referenced in 4 publications.

    • Snow WM  et al. Sex-Specific Effects of Chronic Creatine Supplementation on Hippocampal-Mediated Spatial Cognition in the 3xTg Mouse Model of Alzheimer's Disease. Nutrients 12:N/A (2020). PubMed: 33238473
    • Yu L  et al. The New Application of UHPLC-DAD-TOF/MS in Identification of Inhibitors on ß-Amyloid Fibrillation From Scutellaria baicalensis. Front Pharmacol 10:194 (2019). PubMed: 30936829
    • Hatami A  et al. Familial Alzheimer's Disease Mutations within the Amyloid Precursor Protein Alter the Aggregation and Conformation of the Amyloid-ß Peptide. J Biol Chem 292:3172-3185 (2017). PubMed: 28049728
    • Hatami A  et al. Monoclonal antibodies against Aß42 fibrils distinguish multiple aggregation state polymorphisms in vitro and in Alzheimer disease brain. J Biol Chem 289:32131-43 (2014). PubMed: 25281743

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain lysate)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Brain lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Nov 09 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-beta Amyloid 1-42 antibody [M87] - Conformation-Specific

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (APP-PS1 transgenic mouse brain)
    Permeabilization
    Yes - 0.1 M PBS with 3% Triton X
    Specification
    APP-PS1 transgenic mouse brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Nov 06 2015

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