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The researcher would like to know the fixative used in ICC with ab12266.
Asked on Jun 01 2006
I received the following information from the researcher who tested the antibody ab12266 in ICC: "We routinely use ice-cold methanol fixation for 10 minutes, followed by a brief wash with TBS. We have also used 4% paraformaldehyde for 20 minutes followed by permeabilization with either methanol of 0.5% Triton X-100 in TBS and this also seems to work fine." Please let me know if you need further assistance,
Answered on Jun 05 2006