Overview

  • Product name

    Anandamide (ethanol solution), endogenous cannabinoid
  • Description

    Endogenous cannabinoid
  • Alternative names

    • AEA
    • Arachidonylethanolamide
  • Biological description

    Endogenous cannabinoid, binding to CB1, CB2 and vanilloid receptors. Cannabimimetic in vivo.
  • Purity

    > 98%
  • CAS Number

    94421-68-8
  • Chemical structure

    Chemical Structure

Properties

Images

  • ab81298 staining FAK (phospho Y397) in SK-N-SH cells treated with anandamide (ethanol solution) (ab120087), by ICC/IF. Increase in FAK (phospho Y397) expression correlates with increased concentration of anandamide (ethanol solution), as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120087 (anandamide (ethanol solution)) in ethanol, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81298 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. Membranes are stained in red with WGA.

References

This product has been referenced in:

  • Wang X  et al. Significant neuroprotection against ischemic brain injury by inhibition of the MEK1 protein kinase in mice: exploration of potential mechanism associated with apoptosis. J Pharmacol Exp Ther 304:172-8 (2003). Read more (PubMed: 12490588) »
  • Di Marzo V  et al. Anandamide: some like it hot. Trends Pharmacol Sci 22:346-9 (2001). Read more (PubMed: 11431028) »
  • Pertwee RG Pharmacology of cannabinoid receptor ligands. Curr Med Chem 6:635-64 (1999). Read more (PubMed: 10469884) »
See all 3 Publications for this product

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