Overview

  • Product name

    Anti-ANAPC13 antibody
  • Description

    Rabbit polyclonal to ANAPC13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-70 of Human ANAPC13.

  • Positive control

    • ANAPC13 over-expression lysate; Human stomach tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, 59% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab151216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250. Predicted molecular weight: 8 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the APC13 family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANAPC13 antibody
    • Anaphase-promoting complex subunit 13 antibody
    • APC13 antibody
    • APC13_HUMAN antibody
    • Cyclosome subunit 13 antibody
    • DKFZP566D193 antibody
    • SWM1 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab151216 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of Paraffin embedded Human stomach tissue labeling ANAPC13 with ab151216 at 1/50 dilution.
  • All lanes : Anti-ANAPC13 antibody (ab151216) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)

    Lane 2 : ANAPC13 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells


    Predicted band size: 8 kDa

References

This product has been referenced in:

  • Peng XG  et al. Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease. Exp Ther Med 14:1487-1490 (2017). Read more (PubMed: 28810614) »
See 1 Publication for this product

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