Product nameAnti-Ancient ubiquitous protein 1 antibody
See all Ancient ubiquitous protein 1 primary antibodies
DescriptionRabbit polyclonal to Ancient ubiquitous protein 1
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptice corresponding to a region between residue 426 and 476 of Human Ancient ubiquitous protein 1 (NP_853553.1).
- Hela and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab101984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 53 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome.
Tissue specificityDetected in blood platelets and leukocytes (at protein level). Ubiquitous. Highly expressed in placenta, liver, kidney, skeletal muscle, heart and brain.
Sequence similaritiesBelongs to the AUP1 family.
Contains 1 CUE domain.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- AA589454 antibody
- Ancient ubiquitous protein 1 antibody
- AUP 1 antibody
All lanes : Anti-Ancient ubiquitous protein 1 antibody (ab101984) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Ancient ubiquitous protein 1 with ab101984 at 1/1000 (1µg/ml). Detection: DAB.
Detection of Ancient ubiquitous protein 1 by Western Blot of Immunprecipitate.
Lane 1: ab101984, at 1 µg/ml, staining Ancient ubiquitous protein 1 in HeLa whole cell lysate immunoprecipitated using ab101984 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab101984 has not yet been referenced specifically in any publications.