Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Androgen Receptor [EP670Y] antibody

Good
Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Antigen retrieval step
None
Sample
Hamster Tissue sections (neuronal (amygdala)cells)
Specification
neuronal (amygdala)cells
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% serum & PBS w/ 1% Gelatin/ .3% Triton
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Fixative: 4% paraformaldehyde 15 min incubation in 0.5% Sodium Borohydride, Used avidin/biotin blocking kit after blocking in goat serum prior to primary antibody incubation

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Submitted Mar 26 2014

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