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Synthetic peptide within Human Androgen Receptor aa 1-100 (N terminal). The exact sequence is proprietary.
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab108341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For unpurified use at 1/100 - 1/250.
|WB||1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500.
|ChIP||Use at an assay dependent concentration. PubMed: 23817021|
Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling Androgen receptor with Purified ab108341 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Blocking/Diluting buffer - 5% NFDM/TBST
The androgen receptor variant band detected in 22RV1 cells is reported by PMID: 22315407.
We recommend you to try both RIPA and 1%SDS Hot lysis preparation methods to get desired bands.
Blocking and diluting buffer: 5% NFDM/TBST
Lanes 1 - 2: Merged signal (red and green). Green - ab108341 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108341 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.
Unpurified ab108341, at 1/100, staining Androgen Receptor in LnCaP cells by Immunofluorescence.
Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostatic adenocarcinoma tissue by Immunohistochemistry.
Unpurified ab108341, at 1/250, staining Androgen Receptor in paraffin-embedded Human prostate tissue by Immunohistochemistry.
Unpurified ab108341 showing positive staining in Normal testis tissue.
Unpurified ab108341 showing positive staining in Prostatic carcinoma T3 tissue.
Unpurified ab108341 showing positive staining in Breast carcinoma tissue.
Unpurified ab108341 showing negative staining in Normal liver tissue.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"