Recombinant
RabMAb

Recombinant Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Rabbit recombinant monoclonal Androgen Receptor (AR-V7 specific) antibody [EPR15656]. Validated in WB, IHC and tested in Human. Cited in 9 publication(s). Independently reviewed in 3 review(s).

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
    See all Androgen Receptor (AR-V7 specific) primary antibodies
  • Description

    Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Androgen Receptor(AR-V7 specific) aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: C0JKD3

  • Positive control

    • WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.
  • General notes

    Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101). 

    For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15656
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab198394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099).

We recommend to use 1%SDS Hot lysis prepare method.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance

      Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • Androgen receptor splice variant 3 antibody
      • AR-V7 antibody
      • AR-V7(AR3) antibody
      • AR3 antibody
      • ARv7 antibody
      see all

    Images

    • All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution

      Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
      Lane 2 : PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
      Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
      Lane 4 : Human fetal liver lysate at 10 µg
      Lane 5 : Human prostate lysate at 10 µg
      Lane 6 : 22Rv1 (Human prostate carcinoma cell line) whole cell lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 68 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

       

    • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.

      Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

      Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

      PLoS One. 2011;6(11):e27970. PMID:22114732

       

    • Detection of AR-V7 protein by IHC in 3 breast tumors with high levels of AR-V7 mRNA expression (left). Positive staining was not detected in specimens that do not express AR-V7 mRNA (a representative is shown on the right). Inset images of higher magnification demonstrate strong nuclear staining. Refer to PMID 26554309.

      Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296

      license: https://creativecommons.org/licenses/by/3.0/

       

    • All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution (Purified)

      Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
      Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 68 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

      Negative on cancer cells of human prostate cancer (case#:F11194)

      Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

      Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

      PLoS One. 2011;6(11):e27970. PMID:22114732

    • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

      Negative on cancer cells of human prostate cancer (case#:F121524)

      Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

      Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

      PLoS One. 2011;6(11):e27970. PMID:22114732

    • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

      Negative staining on normal human liver tissue.

      Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

      Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    • Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

      Negative staining on human hepatocellular carcinoma.

      Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

      Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    References

    This product has been referenced in:

    • Chen W  et al. miR-103a-2-5p/miR-30c-1-3p inhibits the progression of prostate cancer resistance to androgen ablation therapy via targeting androgen receptor variant 7. J Cell Biochem 120:14055-14064 (2019). Read more (PubMed: 30963631) »
    • Honda M  et al. Differential expression of androgen receptor variants in hormone-sensitive prostate cancer xenografts, castration-resistant sublines, and patient specimens according to the treatment sequence. Prostate 79:1043-1052 (2019). Read more (PubMed: 30998834) »
    See all 19 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jul 08 2019

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (prostate cancer)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    prostate cancer
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Chiping Qian

    Verified customer

    Submitted Sep 09 2016

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (DU145 Prostate cancer cell line)
    Permeabilization
    Yes - 0.1% v/v TritonX-100
    Specification
    DU145 Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Dr. Dimitra Kalamida

    Verified customer

    Submitted May 30 2016

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (LNCaP clone FCG (ATCC CRL-1740))
    Permeabilization
    No
    Specification
    LNCaP clone FCG (ATCC CRL-1740)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Mr. Vincent Tuminelli

    Verified customer

    Submitted Mar 30 2016

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