Recombinant
RabMAb

Recombinant Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Rabbit recombinant monoclonal Androgen Receptor (AR-V7 specific) antibody [EPR15656]. Validated in WB, IHC and tested in Human. Cited in 9 publication(s). Independently reviewed in 3 review(s).

Reviews (4) Submit a question References (21)

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
    See all Androgen Receptor (AR-V7 specific) primary antibodies

  • Description

    Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Androgen Receptor(AR-V7 specific) aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: C0JKD3

  • Positive control

    • WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.

  • General notes

    Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101). 

    For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15656

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab198394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099).

We recommend to use 1%SDS Hot lysis prepare method.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Relevance

      Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site.

    • Cellular localization

      Nuclear

    • Database links

      • Alternative names

        • Androgen receptor splice variant 3 antibody
        • AR-V7 antibody
        • AR-V7(AR3) antibody
        • AR3 antibody
        • ARv7 antibody
        see all

      Images

      • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution

        Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
        Lane 2 : PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
        Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
        Lane 4 : Human fetal liver lysate at 10 µg
        Lane 5 : Human prostate lysate at 10 µg
        Lane 6 : 22Rv1 (Human prostate carcinoma cell line) whole cell lysate at 10 µg

        Secondary
        All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Predicted band size: 68 kDa
        Observed band size: 80 kDa
        why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        Blocking/Dilution buffer: 5% NFDM/TBST.

         

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

        Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

        Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

        Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.

        Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

        Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

        PLoS One. 2011;6(11):e27970. PMID:22114732

         

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/3.0/.

        Detection of AR-V7 protein by IHC in 3 breast tumors with high levels of AR-V7 mRNA expression (left). Positive staining was not detected in specimens that do not express AR-V7 mRNA (a representative is shown on the right). Inset images of higher magnification demonstrate strong nuclear staining. Refer to PMID 26554309.

        Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296

        license: https://creativecommons.org/licenses/by/3.0/

         

      • Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution (Purified)

        Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
        Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

        Lysates/proteins at 15 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

        Predicted band size: 68 kDa
        Observed band size: 80 kDa why is the actual band size different from the predicted?


        Exposure time: 3 minutes


        This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

        Blocking/Dilution buffer: 5% NFDM/TBST.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

        Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

        Negative on cancer cells of human prostate cancer (case#:F11194)

        Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

        Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

        PLoS One. 2011;6(11):e27970. PMID:22114732

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

        Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

        Negative on cancer cells of human prostate cancer (case#:F121524)

        Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

        Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

        PLoS One. 2011;6(11):e27970. PMID:22114732

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

        Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

        Negative staining on normal human liver tissue.

        Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

        Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

        Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

        Negative staining on human hepatocellular carcinoma.

        Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

        Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Chen W  et al. miR-103a-2-5p/miR-30c-1-3p inhibits the progression of prostate cancer resistance to androgen ablation therapy via targeting androgen receptor variant 7. J Cell Biochem 120:14055-14064 (2019). Read more (PubMed: 30963631) »
      • Honda M  et al. Differential expression of androgen receptor variants in hormone-sensitive prostate cancer xenografts, castration-resistant sublines, and patient specimens according to the treatment sequence. Prostate 79:1043-1052 (2019). Read more (PubMed: 30998834) »
      See all 21 Publications for this product

      Publishing research using ab198394? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Prostate cancer cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      20 µg
      Specification
      Prostate cancer cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 08 2019

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor(AR-V7 specific) antibody [EPR15656]

       Inconclusive
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (prostate cancer)
      Permeabilization
      Yes - 0.2% Triton X-100
      Specification
      prostate cancer
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Chiping Qian

      Verified customer

      Submitted Sep 09 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]

       Excellent
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (DU145 Prostate cancer cell line)
      Permeabilization
      Yes - 0.1% v/v TritonX-100
      Specification
      DU145 Prostate cancer cell line
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Dimitra Kalamida

      Verified customer

      Submitted May 30 2016

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]

       Good
      Abreviews
      This product is known to not work in this application or species.
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (LNCaP clone FCG (ATCC CRL-1740))
      Permeabilization
      No
      Specification
      LNCaP clone FCG (ATCC CRL-1740)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. Vincent Tuminelli

      Verified customer

      Submitted Mar 30 2016

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