Name: Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
Brand: RabMAb
SKU: ab198394
Rating: 4 (4 reviews)

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Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
See all Androgen Receptor (AR-V7 specific) primary antibodies
Description

Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
(Peptide available as ab223099)
Positive control
- WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.
General notes
Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101).

For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15656
Isotype

IgG

Alternative Versions
- Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)
- Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
Compatible Secondaries
Immunizing Peptide (Blocking)
- Human Androgen Receptor (AR-V7 specific) peptide (ab223099)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
IHC-P	Human
WB	Human

Application	Abreviews	Notes
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(1)	1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099). We recommend to use 1%SDS Hot lysis prepare method.

Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099). We recommend to use 1%SDS Hot lysis prepare method.

Application notes

Is unsuitable for ICC/IF.

Relevance

Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site.
Cellular localization

Nuclear
Database links
- SwissProt: C0JKD3 Human
- SwissProt: E7EVX6 Human
Alternative names
- Androgen receptor splice variant 3 antibody
- AR-V7 antibody
- AR-V7(AR3) antibody
- AR3 antibody
- ARv7 antibody
see all

Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution

Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
Lane 2 : PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
Lane 4 : Human fetal liver lysate at 10 µg
Lane 5 : Human prostate lysate at 10 µg
Lane 6 : 22Rv1 (Human prostate carcinoma cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/3.0/.

Detection of AR-V7 protein by IHC in 3 breast tumors with high levels of AR-V7 mRNA expression (left). Positive staining was not detected in specimens that do not express AR-V7 mRNA (a representative is shown on the right). Inset images of higher magnification demonstrate strong nuclear staining. Refer to PMID 26554309.

Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296

license: https://creativecommons.org/licenses/by/3.0/
Western blot - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution (Purified)

Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative on cancer cells of human prostate cancer (case#:F11194)

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative on cancer cells of human prostate cancer (case#:F121524)

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

PLoS One. 2011;6(11):e27970. PMID:22114732

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative staining on normal human liver tissue.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

Negative staining on human hepatocellular carcinoma.

Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab198394? Please let us know so that we can cite the reference in this datasheet.

ab198394 has been referenced in 27 publications.

Cao Q et al. Targeting the KIF4A/AR Axis to Reverse Endocrine Therapy Resistance in Castration-resistant Prostate Cancer. Clin Cancer Res 26:1516-1528 (2020). PubMed: 31796514
Takayama KI et al. Identification of long non-coding RNAs in advanced prostate cancer associated with androgen receptor splicing factors. Commun Biol 3:393 (2020). PubMed: 32704143
Cato L et al. ARv7 Represses Tumor-Suppressor Genes in Castration-Resistant Prostate Cancer. Cancer Cell 35:401-413.e6 (2019). PubMed: 30773341
Chen W et al. miR-103a-2-5p/miR-30c-1-3p inhibits the progression of prostate cancer resistance to androgen ablation therapy via targeting androgen receptor variant 7. J Cell Biochem 120:14055-14064 (2019). PubMed: 30963631
Honda M et al. Differential expression of androgen receptor variants in hormone-sensitive prostate cancer xenografts, castration-resistant sublines, and patient specimens according to the treatment sequence. Prostate 79:1043-1052 (2019). PubMed: 30998834

View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Prostate cancer cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Prostate cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 08 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (prostate cancer)

Permeabilization

Yes - 0.2% Triton X-100

Specification

prostate cancer

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Fixative

Paraformaldehyde

Chiping Qian

Verified customer

Submitted Sep 09 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (DU145 Prostate cancer cell line)

Permeabilization

Yes - 0.1% v/v TritonX-100

Specification

DU145 Prostate cancer cell line

Blocking step

BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C

Fixative

Paraformaldehyde

Dr. Dimitra Kalamida

Verified customer

Submitted May 30 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (LNCaP clone FCG (ATCC CRL-1740))

Permeabilization

Specification

LNCaP clone FCG (ATCC CRL-1740)

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Paraformaldehyde

Mr. Vincent Tuminelli

Verified customer

Submitted Mar 30 2016

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Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Associated products

Alternative Versions

Compatible Secondaries

Immunizing Peptide (Blocking)

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Relevance

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (27)

Customer reviews and Q&As

Western blot abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor(AR-V7 specific) antibody [EPR15656]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]