Recombinant Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific)
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656]
See all Androgen Receptor (AR-V7 specific) primary antibodies -
Description
Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) -
Host species
Rabbit -
Specificity
This target is a splice variant of AR lacking the C-terminal androgen binding site.
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Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab223099) -
Positive control
- WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.
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General notes
Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101).
For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15656 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab198394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB | (1) |
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099).
We recommend to use 1%SDS Hot lysis prepare method. |
Notes |
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IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Androgen Receptor (AR-V7 specific) peptide (ab223099). We recommend to use 1%SDS Hot lysis prepare method. |
Target
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Relevance
Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site. -
Cellular localization
Nuclear -
Database links
- SwissProt: C0JKD3 Human
- SwissProt: E7EVX6 Human
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Alternative names
- Androgen receptor splice variant 3 antibody
- AR-V7 antibody
- AR-V7(AR3) antibody
see all
Images
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All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution
Lane 1 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate at 10 µg
Lane 2 : PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg
Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
Lane 4 : Human fetal liver lysate at 10 µg
Lane 5 : Human prostate lysate at 10 µg
Lane 6 : 22Rv1 (Human prostate carcinoma cell line) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Detection of AR-V7 protein by IHC in 3 breast tumors with high levels of AR-V7 mRNA expression (left). Positive staining was not detected in specimens that do not express AR-V7 mRNA (a representative is shown on the right). Inset images of higher magnification demonstrate strong nuclear staining. Refer to PMID 26554309.
Image from Hickey et al., Oncotarget., 6(42): 44728–44744. Fig 2 (Panel D).; doi: https://doi.org/10.18632/oncotarget.6296
license: https://creativecommons.org/licenses/by/3.0/
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All lanes : Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] (ab198394) at 1/1000 dilution (Purified)
Lane 1 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method
Lane 2 : 22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol here (downloadable copy).
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.
Negative on cancer cells of human prostate cancer (case#:F11194)
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.
Negative on cancer cells of human prostate cancer (case#:F121524)
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
PLoS One. 2011;6(11):e27970. PMID:22114732
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.
Negative staining on normal human liver tissue.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.
Negative staining on human hepatocellular carcinoma.
Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717
Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (43)
ab198394 has been referenced in 43 publications.
- Obst JK et al. Differential Gene Expression Profiles between N-Terminal Domain and Ligand-Binding Domain Inhibitors of Androgen Receptor Reveal Ralaniten Induction of Metallothionein by a Mechanism Dependent on MTF1. Cancers (Basel) 14:N/A (2022). PubMed: 35053548
- Xia L et al. m6A-induced repression of SIAH1 facilitates alternative splicing of androgen receptor variant 7 by regulating CPSF1. Mol Ther Nucleic Acids 28:219-230 (2022). PubMed: 35402071
- Chen D et al. Targeting the radiation-induced ARv7-mediated circNHS/miR-512-5p/XRCC5 signaling with Quercetin increases prostate cancer radiosensitivity. J Exp Clin Cancer Res 41:235 (2022). PubMed: 35918767
- Luo M et al. Tautomycin and enzalutamide combination yields synergistic effects on castration-resistant prostate cancer. Cell Death Discov 8:471 (2022). PubMed: 36446767
- Qu Y et al. Upregulation of circ-ASPH contributes to glioma cell proliferation and aggressiveness by targeting the miR-599/AR/SOCS2-AS1 signaling pathway. Oncol Lett 21:388 (2021). PubMed: 33777211