Recombinant
RabMAb

Recombinant Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491)

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free
    See all Androgen Receptor (AR-V7 specific) primary antibodies
  • Description

    Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Specificity

    This target is a splice variant of AR lacking the C-terminal androgen binding site.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Androgen Receptor(AR-V7 specific) aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: C0JKD3

  • Positive control

    • WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer tissue.
  • General notes

    ab209491 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15656
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab209491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).

Please check the parent abID, ab198394, for more information on dilutions.

Target

  • Relevance

    Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Androgen receptor splice variant 3 antibody
    • AR-V7 antibody
    • AR-V7(AR3) antibody
    • AR3 antibody
    • ARv7 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on cancer cells of Human prostate cancer (case#:F123132) is observed.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative on cancer cells of Human prostate cancer (case#:F11194)

     

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative on cancer cells of Human prostate cancer (case#:F121524)

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative staining on normal Human liver tissue.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with Hematoxylin.

    Negative staining on Human hepatocellular carcinoma.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - Low endotoxin, Azide free (ab209491) + 22RV1 (Human prostate carcinoma cell line) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 68 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

References

ab209491 has not yet been referenced specifically in any publications.

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