Key features and details
- Potent anti-inflammatory agent
- CAS Number: 5508-58-7
- Purity: > 99%
- Soluble in DMSO to 100 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Andrographis paniculata
Product nameAndrographolide, anti-inflammatory agent
DescriptionPotent anti-inflammatory agent
Bioactive component of Andrographis paniculata. Potent anti-inflammatory, immunosuppressant, antithrombotic and antiviral agent. Additionally displays antioxidative stress, neuroprotective and antinociceptive activities.
Storage instructionsStore at +4°C. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM and in ethanol to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab120636, Andrographolide, anti-inflammatory agent
MALME-3M cells were incubated at 37°C for 24h with vehicle control (0 µM) and 1 µM andrographolide (ab120636). Increased expression of p53 in MALME-3M cells correlates with an increase in andrographolide concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab26 at 5 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040 ) at 1/10000 dilution and visualised using ECL development solution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120636 has not yet been referenced specifically in any publications.