• Product name
    Androstenedione ELISA Kit
    See all Androstenedione kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Control Sera 60 <=10%
    Sample n Mean SD CV%
    Control Sera 30 <=9.5%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
  • Sensitivity
    = 0.01 ng/ml
  • Range
    0.1 ng/ml - 10 ng/ml
  • Recovery

    100.91 %

  • Assay duration
    Multiple steps standard assay
  • Product overview

    Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.


    A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Androstenedione Control 1 x 1ml
    Androstenedione Standard 0 – 0 ng/mL 1 x 1ml
    Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml
    Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml
    Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml
    Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml
    Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml
    Androstenedione-HRP Conjugate 1 x 21ml
    Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit
    Cover foils 1 unit
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Alternative names
    • 4 androstenedione


Our Abpromise guarantee covers the use of ab108672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.


  • Representative Standard Curve using ab108672



This product has been referenced in:
  • Shah AB  et al. Elevated androstenedione in young adult but not early adolescent prenatally androgenized female rats. PLoS One 13:e0196862 (2018). Read more (PubMed: 29723293) »
See 1 Publication for this product

Customer reviews and Q&As


Thank you for contacting us and your interest in our products.

The Androstenedione Human ELISA Kit (ab108672) is provided with androstenedione standard which allows you to perform a standard curve to assess that the kit is working as expected and to quantify the level of androstenedione present in your samples.

The kit can be used for a total of 96 tests (including the standard tests). This is product costs €490.00 with a €48.00 shipping charge to Belgium. If ordered today it will be with you within 3 weeks.

I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

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