Key features and details
- Sensitivity: 0.01 ng/ml
- Range: 0.1 ng/ml - 10 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
Product nameAndrostenedione ELISA Kit
See all Androstenedione kits
Intra-assay Sample n Mean SD CV% Control Sera 60 <=10% Inter-assay Sample n Mean SD CV% Control Sera 30 <=9.5%
Sample typeSerum, Hep Plasma, Cit plasma
Sensitivity= 0.01 ng/ml
Range0.1 ng/ml - 10 ng/ml
Assay durationMultiple steps standard assay
Abcam’s Androstenedione in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Androstenedione in serum and plasma.
A 96-well plate has been precoated with anti-Androstenedione IgG. Samples and the Androstenedione-HRP conjugate are added to the wells, where Androstenedione in the sample competes with the added Androstenedione-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Androstenedione in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Androstenedione Control 1 x 1ml Androstenedione Standard 0 – 0 ng/mL 1 x 1ml Androstenedione Standard 1 – 0.1 ng/mL 1 x 1ml Androstenedione Standard 2 – 0.4 ng/mL 1 x 1ml Androstenedione Standard 3 – 1.2 ng/mL 1 x 1ml Androstenedione Standard 4 – 4.0 ng/mL 1 x 1ml Androstenedione Standard 5 – 10.0 ng/mL 1 x 1ml Androstenedione-HRP Conjugate 1 x 21ml Anti-Androstenedione IgG Coated Microplate (12 x 8 wells) 1 unit Cover foils 1 unit Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
- 4 androstenedione
ab108672 has been referenced in 2 publications.
- Shah AB et al. Elevated androstenedione in young adult but not early adolescent prenatally androgenized female rats. PLoS One 13:e0196862 (2018). PubMed: 29723293
- Ronis MJ et al. EB 2017 Article: Soy protein isolate feeding does not result in reproductive toxicity in the pre-pubertal rat testis. Exp Biol Med (Maywood) 243:695-707 (2018). PubMed: 29763383