Our Abpromise guarantee covers the use of ab10600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 14.4 kDa. Non-reducing conditions and overnight incubation is recommended. Reducing conditions can be used but often needs higher amounts of sample.
ICC/IF 1/500.
IHC-P 1/500.
IHC-Fr Use at an assay dependent concentration.


  • Function

    May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
  • Tissue specificity

    Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
  • Involvement in disease

    Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
  • Sequence similarities

    Belongs to the pancreatic ribonuclease family.
  • Developmental stage

    Low level expression in the developing fetus, increased in the neonate, and maximal in the adult.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • ALS9 antibody
    • ANG 1 antibody
    • ANG antibody
    • ANG I antibody
    • ANG1 antibody
    • ANGI antibody
    • ANGI_HUMAN antibody
    • Angiogenin antibody
    • Angiogenin ribonuclease RNase A family, 5 antibody
    • Epididymis luminal protein 168 antibody
    • HEL168 antibody
    • MGC22466 antibody
    • MGC71966 antibody
    • Ribonuclease 5 antibody
    • Ribonuclease RNase A Family 5 antibody
    • RNase 5 antibody
    • RNASE4 antibody
    • RNASE5 antibody
    see all


  • ab10600 staining Angiogenin in murine dermis (including follicle) by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 10% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/500 dilution for 16 hours at 4°C. An Alexa Fluor®555 (red) conjugated donkey anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

    See Abreview

  • Anti-Angiogenin antibody [14017.7] (ab10600) at 2 µg/ml + Recombinant human Angiogenin (E. coli derived) at 1 µg

    Anti-mouse IgG conjugated to HRP at 1/5000 dilution

    Developed using the ECL technique.

    Exposure time: 45 minutes


This product has been referenced in:

  • Farkas C  et al. Streamlined computational pipeline for genetic background characterization of genetically engineered mice based on next generation sequencing data. BMC Genomics 20:131 (2019). Read more (PubMed: 30755158) »
  • Tripolszki K  et al. Angiogenin mutations in Hungarian patients with amyotrophic lateral sclerosis: Clinical, genetic, computational, and functional analyses. Brain Behav 9:e01293 (2019). Read more (PubMed: 31025543) »
See all 8 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A


Thanks for your email. I agree that your blot looks much better. Great idea to change the secondary antibody! It may be that this higher than expected band is non-reduced Angiogenin. I suggest running a reduced and non-reduced sample of each cell line. We would expect that the reduced samples will show a band closer to the predicted molecular weight of Angiogenin. Please note that the reduced samples should not be run adjacent to non-reduced samples as the reducing agent may leak across to the adjacent lane. I look forward to hearing the results of your next western blot. Please let me know if you have any other questions.

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The liver sample extract ab29889 was used as a sample for western blotting where Angiogenin was detected by another anti-Angiogenin antibody, ab95389. This western blot is shown on the datasheet for ab95389 https://www.abcam.com/Angiogenin-antibody-ab95389.html.  Thus it should also work as a positive control for detecing Angiogenin with ab10600. I hope this is helpful. Please contact me again with any further questions.  

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Thank you for your enquiry. I am happy to help you troubleshoot your western blotting experiment. I just have a few additional questions: What is protein sample? (what cell line or tissue and from what species) Have you stained these samples with any other primary antibodies successfully? Have you used a no primary or isotype control blot? Have you successfully used the same secondary antibody for any other blots recently? Should molecular weight markers be so intensely stained? Thanks in advance for the additional information!

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Thank you for your reply and for providing this protocol information.  We will use this to investigate this issue further. Did you purchase the antibody directly from Abcam or from one of our distributors?  I have not been able to find the order number you provided in our system.  Approximately when did you receive the antibody?  I am happy to process your request for a credit. I look forward to your reply so that I may assist you further. 

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Thank you for contacting Abcam regarding ab10600. I am sorry that you have been experiencing difficulties with this antibody in IHC.  This product is guaranteed by our Abpromise for IHC-Fr in mouse tissue.  For our records, I would like to gather some additional information regarding the protocol that you tested.  We will use this information to investigate this issue further. Can you please provide an order number as well as a summary of the protocol conditions tested?  I can then provide either a replacement from a different lot or a credit.  Please let me know which you would prefer.  That replacement would also be guaranteed and eligible for a refund. I look forward to your reply.  Please do not hesitate to contact us if you have any additional questions.

Read More
Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Dermis (including follicle))
Dermis (including follicle)
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 19°C

Abcam user community

Verified customer

Submitted Jan 25 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Rat Tissue sections (brain)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C

Mrs. Cindi Roberts

Verified customer

Submitted Nov 17 2010

Western blot
Human Recombinant protein (Recombinant Human Angiogenin (E. coli derived))
Loading amount
1 µg
Recombinant Human Angiogenin (E. coli derived)
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 19 2010

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