Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18233-116] to Angiogenin
- Suitable for: Sandwich ELISA
- Reacts with: Mouse
Product nameAnti-Angiogenin antibody [EPR18233-116]
See all Angiogenin primary antibodies
DescriptionRabbit monoclonal [EPR18233-116] to Angiogenin
Tested applicationsSuitable for: Sandwich ELISAmore details
Species reactivityReacts with: Mouse
The details of the immunogen for this antibody are not available.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab222525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
FunctionMay function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
Tissue specificityExpressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.
Involvement in diseaseDefects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy.
Sequence similaritiesBelongs to the pancreatic ribonuclease family.
Developmental stageLow level expression in the developing fetus, increased in the neonate, and maximal in the adult.
- Information by UniProt
- ALS9 antibody
- AMYOTROPHIC LATERAL SCLEROSIS antibody
- ANG 1 antibody
Example of mouse Angiogenin standard curve in 1X Cell Extraction Buffer PTR using recombinant Angiogenin RabMAb antibody (ab222525) and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.
For a complete ELISA kit, see Mouse Angiogenin ELISA kit (ab208349).
For a matched antibody pair set, see Mouse Angiogenin Matched Antibody Pair Kit (ab215073).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222525 has not yet been referenced specifically in any publications.