Key features and details
- Rabbit polyclonal to Angiomotin
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Angiomotin antibody
See all Angiomotin primary antibodies
DescriptionRabbit polyclonal to Angiomotin
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
Synthetic peptide, corresponding to a region within amino acids 1034-1084 of Human Angiomotin (NP_001106962.1).
- 293T whole cell lysates.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab117776 was affinity purified using an epitope specific to Angiomotin immobilized on solid support.
Our Abpromise guarantee covers the use of ab117776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 118 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPlays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Tissue specificityExpressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Sequence similaritiesBelongs to the angiomotin family.
DomainThe coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
- Information by UniProt
- AMOT antibody
- AMOT_HUMAN antibody
- Angiomotin antibody
All lanes : Anti-Angiomotin antibody (ab117776) at 0.04 µg/ml
Lane 1 : 293T whole cell lysates at 50 µg
Lane 2 : 293T whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Exposure time: 3 seconds
Detection of Angiomotin by Western Blot of Immunprecipitate.
ab117776 at 1µg/ml staining Angiomotin in 293T whole cell lysate immunoprecipitated using ab117776 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 1 second.
ab117776 has been referenced in 1 publication.
- Nakajima H et al. Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance. Dev Cell 40:523-536.e6 (2017). PubMed: 28350986