Product nameAnti-Angiomotin antibody
See all Angiomotin primary antibodies
DescriptionRabbit polyclonal to Angiomotin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Angiomotin aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Placenta Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).|
FunctionPlays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Tissue specificityExpressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Sequence similaritiesBelongs to the angiomotin family.
DomainThe coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
- Information by UniProt
- AMOT antibody
- AMOT_HUMAN antibody
- Angiomotin antibody
Anti-Angiomotin antibody (ab85143) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Additional bands at: 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 15 minutes
The banding pattern observed is comparable to other commercially available antibodies to Angiomotin. Abcam welcomes customer feedback.
IHC image of Angiomotin staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85143, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Moyon A et al. Early prediction of revascularisation by angiomotin-targeting positron emission tomography. Theranostics 8:4985-4994 (2018). Read more (PubMed: 30429881) »
- Zhu G et al. 12-O-Tetradecanoylphorbol-13-acetate (TPA) is anti-tumorigenic in liver cancer cells via inhibiting YAP through AMOT. Sci Rep 7:44940 (2017). Read more (PubMed: 28322318) »