Anti-Angiomotin antibody (ab85143)

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Key features and details

  • Rabbit polyclonal to Angiomotin
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Angiomotin antibody
    See all Angiomotin primary antibodies

  • Description

    Rabbit polyclonal to Angiomotin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human Angiomotin aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab97422)

  • Positive control

    • This antibody gave a positive signal in Human Placenta Tissue Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skeletal muscle.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab85143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).

Target

  • Function

    Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.

  • Tissue specificity

    Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.

  • Sequence similarities

    Belongs to the angiomotin family.

  • Domain

    The coiled coil domain interacts directly with the BAR domain of ARHGAP17.
    The angiostatin binding domain (871-1005) allows the binding to angiostatin.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
  • Information by UniProt

  • Database links

    • Alternative names

      • AMOT antibody
      • AMOT_HUMAN antibody
      • Angiomotin antibody
      • Angiomotin p130 isoform antibody
      • KIAA1071 antibody
      see all

    Images

    • Western blot - Anti-Angiomotin antibody (ab85143)
      Western blot - Anti-Angiomotin antibody (ab85143)
      Anti-Angiomotin antibody (ab85143) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 118 kDa
      Observed band size: 118 kDa
      Additional bands at: 250 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 15 minutes


      The banding pattern observed is comparable to other commercially available antibodies to Angiomotin. Abcam welcomes customer feedback.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiomotin antibody (ab85143)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiomotin antibody (ab85143)

      IHC image of Angiomotin staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85143, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (4)

    Publishing research using ab85143? Please let us know so that we can cite the reference in this datasheet.

    ab85143 has been referenced in 4 publications.

    • Wang D  et al. Soluble CD146, a cerebrospinal fluid marker for neuroinflammation, promotes blood-brain barrier dysfunction. Theranostics 10:231-246 (2020). PubMed: 31903117
    • Moyon A  et al. Early prediction of revascularisation by angiomotin-targeting positron emission tomography. Theranostics 8:4985-4994 (2018). PubMed: 30429881
    • Zhu G  et al. 12-O-Tetradecanoylphorbol-13-acetate (TPA) is anti-tumorigenic in liver cancer cells via inhibiting YAP through AMOT. Sci Rep 7:44940 (2017). PubMed: 28322318
    • Ma L  et al. Cluster of differentiation 166 (CD166) regulated by phosphatidylinositide 3-Kinase (PI3K)/AKT signaling to exert its anti-apoptotic role via yes-associated protein (YAP) in liver cancer. J Biol Chem 289:6921-33 (2014). WB . PubMed: 24482231

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