Anti-Angiomotin antibody (ab85143)

Anti-Angiomotin antibody (ab85143) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 118 kDa
Observed band size: 118 kDa
Additional bands at: 250 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 15 minutes


The banding pattern observed is comparable to other commercially available antibodies to Angiomotin. Abcam welcomes customer feedback.

IHC image of Angiomotin staining in Human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85143, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.