Key features and details
- Mouse monoclonal [OTI1F11] to Angiomotin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Angiomotin antibody [OTI1F11]
See all Angiomotin primary antibodies
DescriptionMouse monoclonal [OTI1F11] to Angiomotin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Angiomotin aa 1-675. Produced in HEK293T cells (NP_573572).
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Database link: Q4VCS5
- WB: pCMV6-ENTRY Angiomotin cDNA transfected HEK-293T cell lysate.
Clone OTI1F11 (formerly 1F11).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 118 kDa.|
FunctionPlays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Tissue specificityExpressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Sequence similaritiesBelongs to the angiomotin family.
DomainThe coiled coil domain interacts directly with the BAR domain of ARHGAP17.
The angiostatin binding domain (871-1005) allows the binding to angiostatin.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCell junction > tight junction. Localized on the cell surface. May act as a transmembrane protein.
- Information by UniProt
- AMOT antibody
- AMOT_HUMAN antibody
- Angiomotin antibody
All lanes : Anti-Angiomotin antibody [OTI1F11] (ab236498) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY Angiomotin cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 118 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236498 has not yet been referenced specifically in any publications.